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Gaba transport inSaccharomyces cerevisiae
- Source :
- Yeast. 6:263-270
- Publication Year :
- 1990
- Publisher :
- Wiley, 1990.
-
Abstract
- Gamma-aminobutyrate (GABA) accumulation in growing cultures of Saccharomyces cerevisiae was shown to occur by means of an active transport system that is inhibited by proton ionophores, azide, fluoride and arsenate ions. Transport occurred maximally at pH 5.0 and exhibited apparent Km values of 12 microM and 0.1 mM. Accumulated GABA did not efflux upon treatment with proton ionophores and exchanged with extracellular material only very slowly. However, release was complete upon treatment with nystatin. These observations raise the possibility that a major portion of intracellular GABA is sequestered in the vacuole. The response of GABA uptake to growth on various nitrogen sources suggested that uptake may be subject to several types of regulation.
- Subjects :
- Nitrogen
Saccharomyces cerevisiae
Arsenate
Biological Transport, Active
Bioengineering
Vacuole
Hydrogen-Ion Concentration
Biology
biology.organism_classification
Applied Microbiology and Biotechnology
Biochemistry
Yeast
Culture Media
Kinetics
chemistry.chemical_compound
chemistry
Genetics
Azide
Efflux
Proton Ionophores
gamma-Aminobutyric Acid
Intracellular
Biotechnology
Subjects
Details
- ISSN :
- 10970061 and 0749503X
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Yeast
- Accession number :
- edsair.doi.dedup.....3f46d4460544f9030b57375dd4b5dbe8