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Ulinastatin suppresses endoplasmic reticulum stress and apoptosis in the hippocampus of rats with acute paraquat poisoning
- Source :
- Neural Regeneration Research, Vol 10, Iss 3, Pp 467-472 (2015), Neural Regeneration Research
- Publication Year :
- 2015
- Publisher :
- Wolters Kluwer Medknow Publications, 2015.
-
Abstract
- Lung injury is the main manifestation of paraquat poisoning. Few studies have addressed brain damage after paraquat poisoning. Ulinastatin is a protease inhibitor that can effectively stabilize lysosomal membranes, prevent cell damage, and reduce the production of free radicals. This study assumed that ulinastatin would exert these effects on brain tissues that had been poisoned with paraquat. Rat models of paraquat poisoning were intraperitoneally injected with ulinastatin. Simultaneously, rats in the control group were administered normal saline. Hematoxylin-eosin staining showed that most hippocampal cells were contracted and nucleoli had disappeared in the paraquat group. Fewer cells in the hippocampus were concentrated and nucleoli had disappeared in the ulinastatin group. Western blot assay showed that expressions of GRP78 and cleaved-caspase-3 were significantly lower in the ulinastatin group than in the paraquat group. Immunohistochemical findings showed that CHOP immunoreactivity was significantly lower in the ulinastatin group than in the paraquat group. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling staining showed that the number of apoptotic cells was reduced in the paraquat and ulinastatin groups. These data confirmed that endoplasmic reticular stress can be induced by acute paraquat poisoning. Ulinastatin can effectively inhibit this stress as well as cell apoptosis, thereby exerting a neuroprotective effect.
- Subjects :
- hippocampus
paraquat
sciatic nerve
Pharmacology
SMAD3
real-time quantitative PCR
heterocyclic compounds
NSFC grant
neural stem cells
reactive oxygen species
dextromethorphan
ulinastatin
transforming growth factor β1
lipid peroxidation
task difficulty
spiral ganglion cells
diffusion tensor imaging
gene therapy
electroacupuncture therapy
trauma
ototoxicity
superparamagnetic iron oxide
apparent diffusion coefficient
nanocarrier
neuroprotection
DAPT
neural regeneration
malondialdehyde
medicine.medical_specialty
laser photocoagulation
notch signaling pathway
proliferation
Chinese herbal formula
Caspase 3
Brain damage
Neuroprotection
cell transplantation
Cell damage
JNK3
cerebral hemorrhage
Notch1
astrocytes
Xuesai Tong
spinal cord
survival curve
Ulinastatin
medicine.disease
poisoning
glaucoma
chemistry
Apoptosis
regeneration
magnetic guidance
Src kinase
transcranial direct current stimulation
intraocular pressure
caspase-3
type 1 diabetes
cochlea
microglia
cisplatin
neurons
cerebral ischemia
lcsh:RC346-429
neuroinflammation
rosiglitazone
chemistry.chemical_compound
Paraquat
visuomotor coordination
primary motor area
magnetic resonance imaging
dentate gyrus
retinal ganglion cell
ongluo Jiunao injection
nerve regeneration
X-linked inhibitor of apoptosis protein
tamoxifen
Tneurotrophic factor
auranofin
neurodegeneration
average combined score
apoptosis
differentiation
PP2
spinal cord transection
immunohistochemistry
Evans blue tracer
endoplasmic reticulum stress
medicine.symptom
hypothermia
fractional anisotropy
blood-nerve barrier
acupuncture
Research Article
GRP78
Lung injury
fiber tractography
Developmental Neuroscience
medicine
lumbar puncture
peripheral nerve injury
neural degeneration
lcsh:Neurology. Diseases of the nervous system
magnetic resonance image
business.industry
nerve growth factor receptor
bone marrow mesenchymal stem cells
polyethyleneimine-polyethylene glycol
brain injury
spinal cord injury
Surgery
Hes1
rats
Alzheimer′s disease
inflammation
ocular hypertension
business
motor learning
CHOP
western blot assay
Subjects
Details
- Language :
- English
- ISSN :
- 16735374
- Volume :
- 10
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Neural Regeneration Research
- Accession number :
- edsair.doi.dedup.....3f88f2744809232554199bebaed47507