Multi-Omic Single-Shot Technology for Integrated Proteome and Lipidome Analysis

Mass spectrometry (MS) serves as the centerpiece technology for proteome, lipidome, and metabolome analysis. To have a better understanding of the multi-faceted networks of myriad regulatory layers in complex organisms, an integration on different layers of multi-omics is up-and-coming, including joint extraction methods of diverse biomolecular classes and comprehensive data analyses of different omics. Despite the versatility of MS systems, fractured methodology drives nearly all MS laboratories to specialize in analysis of a single ome at the exclusion of the others. Although liquid chromatography-mass spectrometry (LC-MS) analysis is similar for different biomolecular classes, the integration on the instrument level is lagging behind. The recent advancement on high flow proteomics enables us to take a first step on integration of proteins and lipids analysis. Here, we describe a technology to achieve broad and deep coverage of multiple molecular classes simultaneously through multi-omic single-shot technology (MOST) requiring only one column, one LC-MS instrument and a simplified workflow. MOST achieved great robustness and reproducibility. Its application on a Saccharomyces cerevisiae study consisting of 20 conditions revealed 2,842 protein groups and 325 lipids, and potential molecular relationships.