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Establishment of a tumor sphere cell line from a metastatic brain neuroendocrine tumor
- Source :
- Medical molecular morphology. 50(4)
- Publication Year :
- 2017
-
Abstract
- Neuroendocrine tumors are rare, and little is known about the existence of cancer stem cells in this disease. Identification of the tumorigenic population will contribute to the development of effective therapies targeting neuroendocrine tumors. Surgically resected brain metastases from a primary neuroendocrine tumor of unknown origin were dissociated and cultured in serum-free neurosphere medium. Stem cell properties, including self-renewal, differentiation potential, and stem cell marker expression, were examined. Tumor formation was evaluated using intracranial xenograft models. The effect of temozolomide was measured in vitro by cell viability assays. We established the neuroendocrine tumor sphere cell line ANI-27S, which displayed stable exponential growth, virtually unlimited expansion in vitro, and expression of stem-cell markers such as CD133, nestin, Sox2, and aldehyde dehydrogenase. FBS-induced differentiation decreased Sox2 and nestin expression. On the basis of real-time PCR, ANI-27S cells expressed the neuroendocrine markers synaptophysin and chromogranin A. Intracranial xenotransplanted brain tumors recapitulated the original patient tumor and temozolomide exhibited cytotoxic effects on tumor sphere cells. For the first time, we demonstrated the presence of a sphere-forming, stem cell-like population in brain metastases from a primary neuroendocrine tumor. We also demonstrated the potential therapeutic effects of temozolomide for this disease.
- Subjects :
- 0301 basic medicine
Male
Pathology
medicine.medical_specialty
Cell Survival
Primary Cell Culture
Synaptophysin
Neuroendocrine tumors
Stem cell marker
Pathology and Forensic Medicine
Nestin
03 medical and health sciences
Mice
0302 clinical medicine
SOX2
Cancer stem cell
Neurosphere
Spheroids, Cellular
medicine
Biomarkers, Tumor
Temozolomide
Tumor Cells, Cultured
Animals
Humans
AC133 Antigen
Molecular Biology
Antineoplastic Agents, Alkylating
Aged
biology
Brain Neoplasms
SOXB1 Transcription Factors
General Medicine
Aldehyde Dehydrogenase
medicine.disease
Dacarbazine
Neuroendocrine Tumors
030104 developmental biology
030220 oncology & carcinogenesis
biology.protein
Neoplastic Stem Cells
Chromogranin A
Stem cell
Neoplasm Transplantation
Subjects
Details
- ISSN :
- 18601499
- Volume :
- 50
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Medical molecular morphology
- Accession number :
- edsair.doi.dedup.....3fa48db3d232187ccc880cd5927b9424