Back to Search
Start Over
HIF-1α up-regulates NDRG1 expression through binding to NDRG1 promoter, leading to proliferation of lung cancer A549 cells
- Source :
- Molecular biology reports. 40(5)
- Publication Year :
- 2012
-
Abstract
- Hypoxia-inducible signaling pathway is involved in many pathological processes, such as adaptiveness regulation of plateau environment, myocardial ischemia and tumorigenesis. NDRG1 is a member of the N-myc downregulated gene (NDRG) family, and it has strong hypoxia stress reaction functions. Although the cellular responses to hypoxia are well known, little is known about the interaction between hypoxia-inducible transcription factor (HIF)-1α and NDRG1. In this study, we cloned HIF-1α CDS, NDRG1 promoter and its truncatures, constructed pCDNA3.0-Hif-1α and pGL3-basic-NDRG1. Reporter assay results showed that HIF-1α could bind to NDRG1 promoter to activate NDRG1 expression. Further results revealed that -1202 to -450 of NDRG1 promoter is the most important region for HIF-1α binding. Then, we constructed NDRG1 stable transfection cell line. Results from MTT, colony-forming assay and flow cytometry showed that NDRG1 overexpression results in more proliferation and less apoptosis of A549 lung cancer cells. Our study elucidates the mechanism of NGRG1 in hypoxia stress reactions and may provide new strategy for hypoxia injuries.
- Subjects :
- Transcriptional Activation
Lung Neoplasms
Gene Expression
Apoptosis
Cell Cycle Proteins
Biology
medicine.disease_cause
Cell Line, Tumor
Gene expression
Genetics
medicine
Humans
Promoter Regions, Genetic
Molecular Biology
Transcription factor
Cell Proliferation
A549 cell
Regulation of gene expression
Reporter gene
Cell growth
Intracellular Signaling Peptides and Proteins
General Medicine
Hypoxia-Inducible Factor 1, alpha Subunit
Molecular biology
Cell Hypoxia
Cell biology
Up-Regulation
Gene Expression Regulation, Neoplastic
Signal transduction
Carcinogenesis
Protein Binding
Subjects
Details
- ISSN :
- 15734978
- Volume :
- 40
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Molecular biology reports
- Accession number :
- edsair.doi.dedup.....3fdc4ee86373b90dc60bd923cda1073c