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Two supporting factors greatly improve the efficiency of human iPSC generation

Authors :
Han Qin
Tingting Xiang
Yetao Wu
Qiang Zhang
Yiding Zhao
Chengang Xiang
Yanqin Li
Weifeng Yang
Haisong Liu
Mingxiao Ding
Fangfang Zhu
Yang Zhao
Yanxia Liu
Weiqi Zhang
Kai Cui
Meng Liu
Pingping Hou
Honggang Li
Kehu Yuan
Chun Liu
Chen Yu
Hongkui Deng
Xiaolei Yin
Jinning Lou
Xiuxia Qu
Pengbo Zhang
Zhihua Song
Jun Yong
Jun Cai
Source :
Cell stem cell. 3(5)
Publication Year :
2008

Abstract

Summary Human fibroblasts can be induced into pluripotent stem cells (iPSCs), but the reprogramming efficiency is quite low. Here, we screened a panel of candidate factors in the presence of OCT4 , SOX2 , KLF4 , and c-MYC in an effort to improve the reprogramming efficiency from human adult fibroblasts. We found that p53 siRNA and UTF1 enhanced the efficiency of iPSC generation up to 100-fold, even when the oncogene c-MYC was removed from the combinations.

Details

ISSN :
18759777
Volume :
3
Issue :
5
Database :
OpenAIRE
Journal :
Cell stem cell
Accession number :
edsair.doi.dedup.....3ff0bb93200c54f6860e41731596b30c