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Quantitative analysis of glycans, related genes, and proteins in two human bone marrow stromal cell lines using an integrated strategy
- Source :
- Experimental Hematology. 43:760-769.e7
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Altered expression of glycans is associated with cell-cell signal transduction and regulation of cell functions in the bone marrow micro-environment. Studies of this micro-environment often use two human bone marrow stromal cell lines, HS5 and HS27a, co-cultured with myeloid cells. We hypothesized that differential protein glycosylation between these two cell lines may contribute to functional differences in in vitro co-culture models. In this study, we applied an integrated strategy using genomic, proteomic, and functional glycomic techniques for global expression profiling of N-glycans and their related genes and enzymes in HS5 cells versus HS27a cells. HS5 cells had significantly enhanced levels of bisecting N-glycans (catalyzed by MGAT3 [β-1,4-mannosyl-glycoprotein 4-β-N-acetylglucosaminyltransferase]), whereas HS27a cells had enhanced levels of Galβ1,4GlcNAc (catalyzed by β4GalT1 [β4-galactosyltransferase I]). This integrated strategy provides useful information regarding the functional roles of glycans and their related glycogenes and glycosyltransferases in the bone marrow microenvironment, and a basis for future studies of crosstalk among stromal cells and myeloma cells in co-culture.
- Subjects :
- Proteomics
Cancer Research
Stromal cell
Bone Marrow Cells
Biology
N-Acetylglucosaminyltransferases
Article
Glycomics
Cell Line, Tumor
Genetics
medicine
Humans
Molecular Biology
Glycoproteins
Cell Biology
Hematology
Galactosyltransferases
Cell biology
Gene expression profiling
Crosstalk (biology)
medicine.anatomical_structure
Cell culture
Bone marrow
Stromal Cells
Signal transduction
Multiple Myeloma
Subjects
Details
- ISSN :
- 0301472X
- Volume :
- 43
- Database :
- OpenAIRE
- Journal :
- Experimental Hematology
- Accession number :
- edsair.doi.dedup.....40214fedf81fa88640ceade6dc556d86