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Energy transfer with nanoparticles for in vitro diagnostics
- Source :
- Frontiers of Nanoscience, Frontiers of Nanoscience, 16, Elsevier, pp.383, 2020, Colloids for Nanobiotechnology Synthesis, Characterization and Potential Applications, 9780081028285, Frontiers of Nanoscience, 16, Elsevier, pp.383, 2020, Colloids for Nanobiotechnology Synthesis, Characterization and Potential Applications, 9780081028285. ⟨10.1016/B978-0-08-102828-5.00003-6⟩
- Publication Year :
- 2020
- Publisher :
- HAL CCSD, 2020.
-
Abstract
- In vitro diagnostics is an important application for nanoparticles in the life sciences and has attracted continuous research interest. While nanoparticles can be used as single luminescent probes, their unique photophysical properties provide many more advantages when they are combined with other nanoparticles or molecules to form energy transfer pairs. Energy transfer-based assays are homogeneous in nature and are often as simple as “mix and measure,” which has catalyzed their frequent use in clinical diagnostics. Most bioanalytical applications involve Forster resonance energy transfer (FRET), a distance-dependent nonradiative energy transfer from a donor to acceptor chromophore through dipole–dipole interaction. Nanosurface energy transfer (NSET) describes energy transfer to metal nanoparticles. Semiconductor quantum dots, upconversion nanoparticles, gold nanoparticles, and carbon dots are among the most applied nanoparticles in in vitro diagnostics and they have been largely explored in the analysis of disease-related nucleic acids, proteins, and small molecules. The utilizations of various nanoparticles with their intrinsic optical properties have changed the diagnostic horizon substantially by improving the assay simplicity, sensitivity, selectivity, robustness, and multiplexing capacity. In this chapter, we discuss the use of nanoparticles in energy transfer assays for in vitro diagnostics and show their strong potential for advancing the field beyond molecular fluorescent probes.
- Subjects :
- [PHYS]Physics [physics]
Materials science
[SDV]Life Sciences [q-bio]
Nanoparticle
Nanotechnology
02 engineering and technology
Chromophore
010402 general chemistry
021001 nanoscience & nanotechnology
01 natural sciences
Acceptor
Small molecule
Fluorescence
0104 chemical sciences
Förster resonance energy transfer
Colloidal gold
[CHIM]Chemical Sciences
0210 nano-technology
Luminescence
ComputingMilieux_MISCELLANEOUS
Subjects
Details
- Language :
- English
- ISBN :
- 978-0-08-102828-5
- ISBNs :
- 9780081028285
- Database :
- OpenAIRE
- Journal :
- Frontiers of Nanoscience, Frontiers of Nanoscience, 16, Elsevier, pp.383, 2020, Colloids for Nanobiotechnology Synthesis, Characterization and Potential Applications, 9780081028285, Frontiers of Nanoscience, 16, Elsevier, pp.383, 2020, Colloids for Nanobiotechnology Synthesis, Characterization and Potential Applications, 9780081028285. ⟨10.1016/B978-0-08-102828-5.00003-6⟩
- Accession number :
- edsair.doi.dedup.....404cf3414eff73907bea794902139902
- Full Text :
- https://doi.org/10.1016/B978-0-08-102828-5.00003-6⟩