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Amustaline (S-303) treatment inactivates high levels of Chikungunya virus in red-blood-cell components
- Source :
- Vox Sanguinis, Vox Sanguinis, Wiley, 2018, 113 (3), pp.232-241. ⟨10.1111/vox.12626⟩, Vox Sanguinis, 2018, 113 (3), pp.232-241. ⟨10.1111/vox.12626⟩
- Publication Year :
- 2018
- Publisher :
- HAL CCSD, 2018.
-
Abstract
- Background and objectives Chikungunya virus (CHIKV) infections have been reported in all continents, and the potential risk for CHIKV transfusion-transmitted infections (TTIs) was demonstrated by the detection of CHIKV RNA-positive donations in several countries. TTIs can be reduced by pathogen inactivation (PI) of blood products. In this study, we evaluated the efficacy of amustaline and glutathione (S-303/GSH) to inactivate CHIKV in red-blood-cell concentrates (RBCs). Material and methods Red-blood-cells were spiked with high level of CHIKV. Infectious titres and RNA loads were measured before and after PI treatment. Residual CHIKV infectivity was also assessed after five successive cell culture passages. Results The mean CHIKV titres in RBCs before inactivation was 5·81 ± 0·18 log10 50% tissue culture infectious dose (TCID50 )/mL, and the mean viral RNA load was 10·49 ± 0·15 log10 genome equivalent (GEq)/mL. No CHIKV TCID was detected after S-303 treatment nor was replicative CHIKV particles and viral RNA present after five cell culture passages of samples obtained immediately after S-303 treatment. Conclusion Chikungunya virus was previously shown to be inactivated by the PI technology using amotosalen and ultraviolet A light for the treatment of plasma and platelets. This new study demonstrates that S-303/GSH can inactivate high titres of CHIKV in RBCs.
- Subjects :
- Amotosalen
Erythrocytes
Blood Safety
Transfusion-transmissible infections
030204 cardiovascular system & hematology
Biology
medicine.disease_cause
Antiviral Agents
Virus
03 medical and health sciences
0302 clinical medicine
NAT testing
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
medicine
Humans
030212 general & internal medicine
Chikungunya
ComputingMilieux_MISCELLANEOUS
Infectivity
Infectious dose
virus diseases
Hematology
General Medicine
Viral Load
Virology
3. Good health
Red blood cell
pathogen inactivation
Amustaline
medicine.anatomical_structure
Nitrogen Mustard Compounds
Acridines
Chikungunya Fever
Virus Inactivation
Chikungunya virus
Viral load
red cell components
Subjects
Details
- Language :
- English
- ISSN :
- 00429007 and 14230410
- Database :
- OpenAIRE
- Journal :
- Vox Sanguinis, Vox Sanguinis, Wiley, 2018, 113 (3), pp.232-241. ⟨10.1111/vox.12626⟩, Vox Sanguinis, 2018, 113 (3), pp.232-241. ⟨10.1111/vox.12626⟩
- Accession number :
- edsair.doi.dedup.....405f0ee6be7389a7d3de96ed165e6ba2