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Isolation and sequence of the Candida albicans FAS1 gene
- Source :
- Gene. 147:119-124
- Publication Year :
- 1994
- Publisher :
- Elsevier BV, 1994.
-
Abstract
- The gene (FAS1) encoding the β-subunit of fatty-acid synthase (FAS) of Candida albicans has been isolated and analyzed. The gene was isolated on the basis of homology to the Saccharomyces cerevisiaeFAS1 gene. Sequence analysis showed that the gene contained an intron-free open reading frame of 6111 bp encoding a protein of 2037 amino acids (aa) (227 916 Da). C. albicansFAS1 and its product exhibit a high degree of overall sequence relatedness to their counterparts in S. cerevisiae, with identities of 68 and 63% at the nucleotide (nt) and aa level, respectively. In addition, the β-subunits of both organisms contain the catalytic domains in an identical sequential order. Northern blots demonstrated that the gene encodes a single mRNA of approx. 6.1 kb, and that changes in transcript levels are not a prerequisite of the yeast-to-hyphal transition. Southern blot analysis of C. albicans chromosomes separated by pulsed-field gel electrophoresis showed that FAS1 resides on chromosome 5.
- Subjects :
- Sequence analysis
Genes, Fungal
Molecular Sequence Data
Gene Expression
Homology (biology)
Candida albicans
Genetics
Amino Acid Sequence
RNA, Messenger
Northern blot
Cloning, Molecular
Gene
Southern blot
Base Sequence
Sequence Homology, Amino Acid
biology
Chromosome Mapping
RNA, Fungal
General Medicine
biology.organism_classification
Molecular biology
Corpus albicans
Open reading frame
Biochemistry
Fatty Acid Synthases
Sequence Alignment
Subjects
Details
- ISSN :
- 03781119
- Volume :
- 147
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....4067e678d66dbe38c333a872284a1e99
- Full Text :
- https://doi.org/10.1016/0378-1119(94)90050-7