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Isolation and sequence of the Candida albicans FAS1 gene

Authors :
Ronald L. Cihlar
Xiao-jiong Zhao
Source :
Gene. 147:119-124
Publication Year :
1994
Publisher :
Elsevier BV, 1994.

Abstract

The gene (FAS1) encoding the β-subunit of fatty-acid synthase (FAS) of Candida albicans has been isolated and analyzed. The gene was isolated on the basis of homology to the Saccharomyces cerevisiaeFAS1 gene. Sequence analysis showed that the gene contained an intron-free open reading frame of 6111 bp encoding a protein of 2037 amino acids (aa) (227 916 Da). C. albicansFAS1 and its product exhibit a high degree of overall sequence relatedness to their counterparts in S. cerevisiae, with identities of 68 and 63% at the nucleotide (nt) and aa level, respectively. In addition, the β-subunits of both organisms contain the catalytic domains in an identical sequential order. Northern blots demonstrated that the gene encodes a single mRNA of approx. 6.1 kb, and that changes in transcript levels are not a prerequisite of the yeast-to-hyphal transition. Southern blot analysis of C. albicans chromosomes separated by pulsed-field gel electrophoresis showed that FAS1 resides on chromosome 5.

Details

ISSN :
03781119
Volume :
147
Database :
OpenAIRE
Journal :
Gene
Accession number :
edsair.doi.dedup.....4067e678d66dbe38c333a872284a1e99
Full Text :
https://doi.org/10.1016/0378-1119(94)90050-7