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Quantitative definition of neurobehavior, vision, hearing and brain volumes in macaques congenitally exposed to Zika virus
- Source :
- PLoS ONE, PLoS ONE, Vol 15, Iss 10, p e0235877 (2020)
- Publication Year :
- 2020
- Publisher :
- Public Library of Science, 2020.
-
Abstract
- Congenital Zika virus (ZIKV) exposure results in a spectrum of disease ranging from severe birth defects to delayed onset neurodevelopmental deficits. ZIKV-related neuropathogenesis, predictors of birth defects, and neurodevelopmental deficits are not well defined in people. Here we assess the methodological and statistical feasibility of a congenital ZIKV exposure macaque model for identifying infant neurobehavior and brain abnormalities that may underlie neurodevelopmental deficits. We inoculated five pregnant macaques with ZIKV and mock-inoculated one macaque in the first trimester. Following birth, growth, ocular structure/function, brain structure, hearing, histopathology, and neurobehavior were quantitatively assessed during the first week of life. We identified the typical pregnancy outcomes of congenital ZIKV infection, with fetal demise and placental abnormalities. We estimated sample sizes needed to define differences between groups and demonstrated that future studies quantifying brain region volumes, retinal structure, hearing, and visual pathway function require a sample size of 14 animals per group (14 ZIKV, 14 control) to detect statistically significant differences in at least half of the infant exam parameters. Establishing the parameters for future studies of neurodevelopmental outcomes following congenital ZIKV exposure in macaques is essential for robust and rigorous experimental design.
- Subjects :
- Sensory Receptors
Maternal Health
Placenta
Social Sciences
Disease
Monkeys
Pathology and Laboratory Medicine
Zika virus
0302 clinical medicine
Animal Cells
Pregnancy
Psychology
Hearing Disorders
Mammals
Neurons
Zika Virus Infection
Delayed onset
Pregnancy Outcome
Eukaryota
Bioassays and Physiological Analysis
Medical Microbiology
Viral Pathogens
Prenatal Exposure Delayed Effects
Medicine
Cellular Types
Macaque
Primates
medicine.medical_specialty
Imaging Techniques
Science
Vision Disorders
Neurophysiology
Nervous System Malformations
Microbiology
03 medical and health sciences
biology.animal
Microbial Pathogens
Flaviviruses
Organisms
Reproductive System
medicine.disease
030104 developmental biology
Eyes
Histopathology
Clinical Medicine
030217 neurology & neurosurgery
Neuroscience
Developmental Biology
0301 basic medicine
RNA viruses
Photoreceptors
Pediatrics
Embryology
Future studies
Vision
Physiology
Medicine and Health Sciences
Pregnancy Complications, Infectious
Clinical Neurophysiology
Brain Mapping
Multidisciplinary
biology
Obstetrics and Gynecology
Electroencephalography
Electrophysiology
Brain Electrophysiology
Vertebrates
Viruses
Fetal Demise
Neuropathogenesis
Sensory Perception
Female
Pathogens
Anatomy
Research Article
Signal Transduction
Neuroimaging
Research and Analysis Methods
Ocular System
Old World monkeys
medicine
Animals
Biology and life sciences
business.industry
Electrophysiological Techniques
Visual-Evoked Potentials
Cognitive Psychology
Afferent Neurons
Zika Virus
Cell Biology
biology.organism_classification
Macaca mulatta
Retinal structure
Animals, Newborn
Cellular Neuroscience
Amniotes
Cognitive Science
Women's Health
Perception
business
Zoology
Head
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 15
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....406c54a7555e7935dac9625dfcd72b67