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Molecular Phylogenetic Diversity and Biological Characterization of Diaporthe Species Associated with Leaf Spots of Camellia sinensis in Taiwan

Authors :
Jaturong Kumla
Medsaii Tanjira
Jian Yuan Wang
Nakarin Suwannarach
Abdallah M. Elgorban
Ratchadawan Cheewangkoon
Hiran A. Ariyawansa
Patchareeya Withee
Ichen Tsai
Shiou Ruei Lin
Source :
Plants, Volume 10, Issue 7, Plants, Vol 10, Iss 1434, p 1434 (2021)
Publication Year :
2021
Publisher :
Multidisciplinary Digital Publishing Institute, 2021.

Abstract

Camellia sinensis is one of the major crops grown in Taiwan and has been widely cultivated around the island. Tea leaves are prone to various fungal infections, and leaf spot is considered one of the major diseases in Taiwan tea fields. As part of a survey on fungal species causing leaf spots on tea leaves in Taiwan, 19 fungal strains morphologically similar to the genus Diaporthe were collected. ITS (internal transcribed spacer), tef1-α (translation elongation factor 1-α), tub2 (beta-tubulin), and cal (calmodulin) gene regions were used to construct phylogenetic trees and determine the evolutionary relationships among the collected strains. In total, six Diaporthe species, including one new species, Diaporthe hsinchuensis, were identified as linked with leaf spot of C. sinensis in Taiwan based on both phenotypic characters and phylogeny. These species were further characterized in terms of their pathogenicity, temperature, and pH requirements under laboratory conditions. Diaporthe tulliensis, D. passiflorae, and D. perseae were isolated from C. sinensis for the first time. Furthermore, pathogenicity tests revealed that, with wound inoculation, only D. hongkongensis was pathogenic on tea leaves. This investigation delivers the first assessment of Diaporthe taxa related to leaf spots on tea in Taiwan.

Details

Language :
English
ISSN :
22237747
Database :
OpenAIRE
Journal :
Plants
Accession number :
edsair.doi.dedup.....407e613b15f8c4a4555a9817b3f3f237
Full Text :
https://doi.org/10.3390/plants10071434