Additional file 3 of Effects of hyperinsulinemia on pancreatic cancer development and the immune microenvironment revealed through single-cell transcriptomics

Additional file 3: Fig. S1. Amylase activity and pancreatic area of PK-Ins1-/-;Ins2+/+ and PK-Ins1-/-;Ins2+/- mice and the sex difference in PanIN development. A-B The amylase activity in male (A) and female mice (B) for each genotype. C-D The comparison of percent of total pancreatic area occupied by PanINs and tumor (C) or only PanINs (excluding tumor bearing mice) (D) in male and female PK-Ins1-/-; Ins2+/+ mice (n= 15-22) (dark blue and dark orange dots denote mice that developed tumors). E The comparison of percent of total pancreatic area occupied by only PanINs in male and female PK-Ins1-/-; Ins2+/- mice (n= 10-14). F The comparison of percent of total pancreatic area occupied by ADM in male and female PK-Ins1-/-; Ins2+/+ mice (n= 16-22). G The comparison of percent of total pancreatic area occupied by ADM in male and female PK-Ins1-/-; Ins2+/- mice (n= 10-14). H The total pancreatic area for mice in an Ins2-null background or in an Ins1-null background. I-J Correlations of adipocyte area with PanIN area in non-tumor bearing male (I) and female (J) PK-Ins1-/-;Ins2+/+ (dark colors) and PK-Ins1-/-;Ins2+/- (light colors) mice (n = 10-22). ****p