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Lichen Secondary Metabolite, Physciosporin, Inhibits Lung Cancer Cell Motility
- Source :
- PLoS ONE, PLOS ONE(10): 9, PLoS ONE, Vol 10, Iss 9, p e0137889 (2015)
- Publication Year :
- 2015
-
Abstract
- Lichens produce various unique chemicals that can be used for pharmaceutical purposes. To screen for novel lichen secondary metabolites showing inhibitory activity against lung cancer cell motility, we tested acetone extracts of 13 lichen samples collected in Chile. Physciosporin, isolated from Pseudocyphellaria coriacea (Hook f. & Taylor) D.J. Galloway & P. James, was identified as an effective compound and showed significant inhibitory activity in migration and invasion assays against human lung cancer cells. Physciosporin treatment reduced both protein and mRNA levels of N-cadherin with concomitant decreases in the levels of epithelial-mesenchymal transition markers such as snail and twist. Physciosporin also suppressed KITENIN (KAI1 C-terminal interacting tetraspanin)-mediated AP-1 activity in both the absence and presence of epidermal growth factor stimulation. Quantitative real-time PCR analysis showed that the expression of the metastasis suppressor gene, KAI1, was increased while that of the metastasis enhancer gene, KITENIN, was dramatically decreased by physciosporin. Particularly, the activity of 3'-untranslated region of KITENIN was decreased by physciosporin. Moreover, Cdc42 and Rac1 activities were decreased by physciosporin. These results demonstrated that the lichen secondary metabolite, physciosporin, inhibits lung cancer cell motility through novel mechanisms of action.
- Subjects :
- rho GTP-Binding Proteins
Epithelial-Mesenchymal Transition
Lung Neoplasms
Lichens
lcsh:Medicine
Motility
RAC1
Antineoplastic Agents
Secondary metabolite
Biology
Kangai-1 Protein
Metastasis
Acetone
Tetraspanin
Cell Movement
Cell Line, Tumor
medicine
Humans
Neoplasm Invasiveness
Epithelial–mesenchymal transition
Neoplasm Metastasis
lcsh:Science
Multidisciplinary
Plant Extracts
lcsh:R
Membrane Proteins
medicine.disease
Molecular biology
Gene Expression Regulation, Neoplastic
Transcription Factor AP-1
Metastasis Suppressor Gene
Cell culture
Immunology
Oxepins
lcsh:Q
Carrier Proteins
medicine.drug
Research Article
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 10
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....40b84debf4e9e304a922a4933a2401ce