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Laminin and Platelet-Derived Growth Factor-BB Promote Neuronal Differentiation of Human Urine-Derived Stem Cells

Laminin and Platelet-Derived Growth Factor-BB Promote Neuronal Differentiation of Human Urine-Derived Stem Cells

Authors :
Jun Nyung Lee
Jung Yeon Kim
Jae-Wook Chung
Jin-Sung Park
Tae Gyun Kwon
So Young Chun
Yun-Sok Ha
Source :
Tissue Engineering and Regenerative Medicine. 15:195-209
Publication Year :
2017
Publisher :
Springer Science and Business Media LLC, 2017.

Abstract

Urine-derived stem cells (USCs) are considered as a promising cell source capable of neuronal differentiation. In addition, specific growth factors and extracellular matrix are essential for enhancing their neuronal differentiation efficiency. In this study, we investigated the possibility of neuronal differentiation of USCs and the role of laminin and platelet-derived growth factor BB (PDGF-BB) as promoting factors. USCs were isolated from fresh urine of healthy donors. Cultured USCs were adherent to the plate and their morphology was similar to the cobblestone. In addition, they showed chromosome stability, rapid proliferation rate, colony forming capacity, and mesenchymal stem cell characteristics. For inducing the neuronal differentiation, USCs were cultured for 14 days in neuronal differentiation media supplemented with/without laminin and/or PDGF-BB. To identify the expression of neuronal markers, RT-PCR, flow cytometry analysis and immunocytochemistry were used. After neuronal induction, the cells showed neuron-like morphological change and high expression level of neuronal markers. In addition, laminin and PDGF-BB respectively promoted the neuronal differentiation of USCs and the combination of laminin and PDGF-BB showed a synergistic effect for the neuronal differentiation of USCs. In conclusion, USCs are noteworthy cell source in the field of neuronal regeneration and laminin and PDGF-BB promote their neuronal differentiation efficiency.

Details

ISSN :
22125469 and 17382696
Volume :
15
Database :
OpenAIRE
Journal :
Tissue Engineering and Regenerative Medicine
Accession number :
edsair.doi.dedup.....40d2f1de1e8f9539025d63cc50f6076a
Full Text :
https://doi.org/10.1007/s13770-017-0102-x