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Laminin and Platelet-Derived Growth Factor-BB Promote Neuronal Differentiation of Human Urine-Derived Stem Cells
Laminin and Platelet-Derived Growth Factor-BB Promote Neuronal Differentiation of Human Urine-Derived Stem Cells
- Source :
- Tissue Engineering and Regenerative Medicine. 15:195-209
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Urine-derived stem cells (USCs) are considered as a promising cell source capable of neuronal differentiation. In addition, specific growth factors and extracellular matrix are essential for enhancing their neuronal differentiation efficiency. In this study, we investigated the possibility of neuronal differentiation of USCs and the role of laminin and platelet-derived growth factor BB (PDGF-BB) as promoting factors. USCs were isolated from fresh urine of healthy donors. Cultured USCs were adherent to the plate and their morphology was similar to the cobblestone. In addition, they showed chromosome stability, rapid proliferation rate, colony forming capacity, and mesenchymal stem cell characteristics. For inducing the neuronal differentiation, USCs were cultured for 14 days in neuronal differentiation media supplemented with/without laminin and/or PDGF-BB. To identify the expression of neuronal markers, RT-PCR, flow cytometry analysis and immunocytochemistry were used. After neuronal induction, the cells showed neuron-like morphological change and high expression level of neuronal markers. In addition, laminin and PDGF-BB respectively promoted the neuronal differentiation of USCs and the combination of laminin and PDGF-BB showed a synergistic effect for the neuronal differentiation of USCs. In conclusion, USCs are noteworthy cell source in the field of neuronal regeneration and laminin and PDGF-BB promote their neuronal differentiation efficiency.
- Subjects :
- 0301 basic medicine
medicine.diagnostic_test
biology
Chemistry
Growth factor
medicine.medical_treatment
Cell
Immunocytochemistry
Mesenchymal stem cell
Biomedical Engineering
Medicine (miscellaneous)
Flow cytometry
Cell biology
Extracellular matrix
03 medical and health sciences
030104 developmental biology
medicine.anatomical_structure
nervous system
Laminin
medicine
biology.protein
Original Article
Stem cell
Subjects
Details
- ISSN :
- 22125469 and 17382696
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Tissue Engineering and Regenerative Medicine
- Accession number :
- edsair.doi.dedup.....40d2f1de1e8f9539025d63cc50f6076a
- Full Text :
- https://doi.org/10.1007/s13770-017-0102-x