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Bisphenol A alternatives bisphenol S and bisphenol F interfere with thyroid hormone signaling pathway in vitro and in vivo
- Source :
- Environmental Pollution. 237:1072-1079
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- The wide use of the alternatives to bisphenol A (BPA) has raised concerns about their potential toxicities. Considering the disrupting activity of BPA on thyroid hormone (TH) signaling, we investigated whether bisphenol S (BPS) and bisphenol F (BPF), two leading alternatives, could interfere with TH signaling pathway using a series of assays in vitro and in vivo. In the fluorescence competitive binding assay, we found BPS and BPF, like BPA, bound to TH receptors (TRα and TRβ), with the binding potencies an order of magnitude lower than BPA (BPA > BPF > BPS). Molecular docking data also show their binding potencies to TRs. In the coactivator recruitment assay, BPS and BPF recruited coactivator to TRβ but not TRα, with weaker potencies than BPA. Correspondingly, agonistic actions of the three bisphenols in the absence or presence of T3 were observed in the TR-mediated reporter gene transcription assay. Also, all the three bisphenols induced TH-dependent GH3 cell proliferation, whereas BPA and BPF inhibited T3 induction in the presence of T3. As for in vivo assay, the three bisphenols like T3 induced TH-response gene transcription in Pelophylax nigromaculatus tadpoles, but in the presence of T3 altered T3-induced gene transcription in a biphasic concentration-response manner. These results for the first time demonstrate that BPS and BPF, like BPA, have potential to interfere with TH signaling pathway, i.e., they generally activate TH signaling in the absence of T3, but in the presence of TH, display agonistic or/and antagonistic actions under certain condition. Our study highlights the potential risks of BPS and BPF as BPA alternatives.
- Subjects :
- 0301 basic medicine
Thyroid Hormones
endocrine system
Bisphenol A
medicine.medical_specialty
Health, Toxicology and Mutagenesis
010501 environmental sciences
Toxicology
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Phenols
Genes, Reporter
In vivo
Internal medicine
Coactivator
medicine
Sulfones
Benzhydryl Compounds
Receptor
0105 earth and related environmental sciences
Reporter gene
urogenital system
General Medicine
Pollution
Molecular Docking Simulation
030104 developmental biology
Endocrinology
chemistry
Bisphenol S
Biological Assay
Signal transduction
Water Pollutants, Chemical
hormones, hormone substitutes, and hormone antagonists
Signal Transduction
Hormone
Subjects
Details
- ISSN :
- 02697491
- Volume :
- 237
- Database :
- OpenAIRE
- Journal :
- Environmental Pollution
- Accession number :
- edsair.doi.dedup.....40df968156f6b4078a8ca3806c46bfda