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Molecular Mechanism of Biofilm Locator Protein Kinase Dbf2p-related kinase 1 in Regulating Innate Immune Response to Interleukin 17-induced Viral Pneumonia
- Source :
- Bioengineered, article-version (VoR) Version of Record, Bioengineered, Vol 12, Iss 2, Pp 10335-10344 (2021)
- Publication Year :
- 2021
-
Abstract
- It focused on the antiviral immune regulation of biofilm-localized protein kinase Dbf2p-related kinase 1 (NDR1) in viral pneumonia. Mouse alveolar monocyte RAW264.7 was used as blank control, and viral pneumonia cell model was prepared by infecting cells with respiratory syncytial virus (RSV). NDR1 overexpression vector and siRNA interference sequences were synthesized, and overexpression/silence NDR1 cell model was fabricated. About 50 ng/mL interleukin 17 (IL-17) was given to stimulate. Enzyme-linked immunosorbent assay (ELISA), quantitative reverse transcription PCR (RT-qRCR), and Western blot were performed to detect cytokines and chemokines, mRNA of inflammatory factors, and signal molecule protein expression. Notably, RSV infection increased RSV-F mRNA in RAW264.7 cells and reduced NDR1 mRNA and protein. Secretion levels of IL-6, interferon β (IFN-β), chemokine (C-X-C motif) ligand 2 (CXCL2), and chemokine (C-C motif) ligand 2 (CCL20) increased in the model group versus blank control (P 0.05). In short, NDR1 regulated innate immune response to viral pneumonia induced by IL-17, which can be used as a new target for the treatment of IL-17-induced inflammatory response and autoimmune diseases.
- Subjects :
- Chemokine
respiratory syncytial virus
Pneumonia, Viral
Bioengineering
Respiratory Syncytial Virus Infections
Protein Serine-Threonine Kinases
Applied Microbiology and Biotechnology
immune response
Mice
Immune system
inflammatory responses
medicine
Animals
Protein kinase A
Cell Proliferation
Inflammation
Innate immune system
biology
Chemistry
Interleukin-17
IL17
NDR1
General Medicine
medicine.disease
RAW264.7
Molecular biology
Immunity, Innate
Respiratory Syncytial Viruses
CCL20
CXCL2
RAW 264.7 Cells
Viral pneumonia
Biofilms
biology.protein
Interleukin 17
Chemokines
TP248.13-248.65
Biotechnology
Signal Transduction
Research Article
Research Paper
Subjects
Details
- ISSN :
- 21655987
- Volume :
- 12
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Bioengineered
- Accession number :
- edsair.doi.dedup.....40e8b378397c3a3191c093bff3b61c66