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Detection and isolation ofToxoplasma gondiifrom fresh semen of naturally infected dogs in Southern Brazil
- Source :
- Reproduction in Domestic Animals. 51:550-554
- Publication Year :
- 2016
- Publisher :
- Wiley, 2016.
-
Abstract
- The aim of this study was to isolate Toxoplasma gondii and determine the viability of the parasite in fresh semen samples of clinically healthy adult dogs naturally infected. Eleven seropositive dogs with T. gondii IgG antibodies from southern Brazil were selected to confirm the presence and viability of T. gondii in fresh semen samples using in vitro isolation in Vero cell culture, polymerase chain reaction (PCR) and sequencing analysis. The presence of viable T. gondii was confirmed by in vitro isolation and PCR in five semen samples. The ITS1 region of the isolated protozoa (TG S4) was amplified and sequenced. The nucleotide sequence obtained was 99% compatible with the T. gondii DNA sequences stored in the GenBank. It has been shown that T. gondii tachyzoites may be isolated in vitro from fresh semen samples of clinically healthy dogs seropositive for T. gondii.
- Subjects :
- 0301 basic medicine
030231 tropical medicine
Semen
Polymerase Chain Reaction
law.invention
03 medical and health sciences
Dogs
0302 clinical medicine
Endocrinology
law
parasitic diseases
Animals
Parasite hosting
Dog Diseases
Polymerase chain reaction
biology
Toxoplasma gondii
DNA, Protozoan
030108 mycology & parasitology
biology.organism_classification
Isolation (microbiology)
Virology
Toxoplasmosis, Animal
biology.protein
Vero cell
Protozoa
Animal Science and Zoology
Antibody
Toxoplasma
Brazil
Biotechnology
Subjects
Details
- ISSN :
- 09366768
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- Reproduction in Domestic Animals
- Accession number :
- edsair.doi.dedup.....40ff412635961e8171033f891d7022c8