Back to Search
Start Over
Elevated Expression of RIOK1 Is Correlated with Breast Cancer Hormone Receptor Status and Promotes Cancer Progression
- Source :
- Cancer Research and Treatment : Official Journal of Korean Cancer Association
- Publication Year :
- 2020
- Publisher :
- Korean Cancer Association, 2020.
-
Abstract
- Purpose RIOK1 has been proved to play an important role in cancer cell proliferation and migration in various types of cancers-such as colorectal and gastric cancers. However, the expression of RIOK1 in breast cancer (BC) and the relationship between RIOK1 expression and the development of BC are not well characterized. In this study, we assessed the expression of RIOK1 in BC and evaluated the mechanisms underlying its biological function in this disease context. Materials and methods We used immunohistochemistry, western blot and quantitative Real-Time polymerase chain reaction (qRT-PCR) to evaluate the expression of RIOK1 in BC patients. Then, knockdown or overexpression of RIOK1 were used to evaluate the effect on BC cells in vitro and in vivo. Finally, we predicted miR-204-5p could be a potential regulator of RIOK1. Results We found that the expression levels of RIOK1 were significantly higher in hormone receptor (HR)-negative BC patients and was associated with tumor grades (p=0.01) and p53 expression (p=0.008) and survival duration (p=0.011). Kaplan-Meier analysis suggested a tendency for the poor prognosis. In vitro, knockdown of RIOK1 could inhibit proliferation, invasion, and induced apoptosis in HR-negative BC cells and inhibited tumorigenesis in vivo, while overexpression of RIOK1 promoted HR-positive tumor progression. MiR-204-5p could regulate RIOK1 expression and be involved in BC progression. Conclusion These findings indicate that RIOK1 expression could be a biomarker of HR-negative BC, and it may serve as an effective prognostic indicator and promote BC progression.
- Subjects :
- 0301 basic medicine
RIOK1
Cancer Research
Apoptosis
Kaplan-Meier Estimate
medicine.disease_cause
PI3K
Mice
0302 clinical medicine
Cell Movement
Breast
Mastectomy
Gene knockdown
miR-204
Middle Aged
Prognosis
Immunohistochemistry
Gene Expression Regulation, Neoplastic
Receptors, Estrogen
Oncology
Hormone receptor
Gene Knockdown Techniques
030220 oncology & carcinogenesis
Disease Progression
Biomarker (medicine)
Female
Original Article
Receptors, Progesterone
Breast Neoplasms
Protein Serine-Threonine Kinases
03 medical and health sciences
Breast cancer
Cell Line, Tumor
Breast Cancer
Biomarkers, Tumor
medicine
Animals
Humans
Neoplasm Invasiveness
PI3K/AKT/mTOR pathway
Cell Proliferation
business.industry
Computational Biology
Cancer
medicine.disease
Xenograft Model Antitumor Assays
MicroRNAs
030104 developmental biology
Tumor progression
Cancer research
Neoplasm Grading
Carcinogenesis
business
Subjects
Details
- ISSN :
- 20059256 and 15982998
- Database :
- OpenAIRE
- Journal :
- Cancer Research and Treatment
- Accession number :
- edsair.doi.dedup.....410a1a881c9ed2f8a2e2ccddf77abd20