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Aldolase C is a novel molecular marker for folliculo-stellate cells in rodent pituitary
- Source :
- Cell and Tissue Research. 381:273-284
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- The anterior pituitary gland is composed of five types of hormone-producing cells and folliculo-stellate cells. Folliculo-stellate cells do not produce anterior pituitary hormones but they are thought to play important roles as stem cells, phagocytes, or supporting cells of hormone-producing cells in the anterior pituitary. S100β protein has been used as a folliculo-stellate cell marker in some animals, including rats. However, since no reliable molecular marker for folliculo-stellate cells has been reported in mice, genetic approaches for the investigation of folliculo-stellate cells in mice are not yet available. Aldolase C/Zebrin II is a brain-type isozyme and is a fructose-1,6-bisphosphate aldolase. In the present study, we first used immunohistochemistry to verify that aldolase C was produced in the anterior pituitary of rats. Moreover, using transgenic rats expressing green fluorescent protein under the control of the S100β gene promoter, we identified aldolase C-immunoreactive signals in folliculo-stellate cells and marginal cells located in the parenchyma of the anterior pituitary and around Rathke's cleft, respectively. We also identified aldolase C-expressing cells in the mouse pituitary using immunohistochemistry and in situ hybridization. Aldolase C was not produced in any pituitary hormone-producing cells, while aldolase C-immunopositive signal co-localized with E-cadherin- and SOX2-positive cells. Using post-embedding immunoelectron microscopy, aldolase C-immunoreactive products were observed in the cytoplasm of marginal cells and folliculo-stellate cells of the mouse pituitary. Taken together, aldolase C is a common folliculo-stellate cell marker in the anterior pituitary gland of rodents.
- Subjects :
- Male
0301 basic medicine
endocrine system
Histology
Immunoelectron microscopy
Nerve Tissue Proteins
In situ hybridization
Pathology and Forensic Medicine
Mice
03 medical and health sciences
0302 clinical medicine
Anterior pituitary
Pituitary Gland, Anterior
Fructose-Bisphosphate Aldolase
Parenchyma
medicine
Animals
biology
Aldolase C
Aldolase A
Cell Biology
Molecular biology
Rats
Mice, Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
Hepatic stellate cell
biology.protein
Rats, Transgenic
Stem cell
Biomarkers
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 14320878 and 0302766X
- Volume :
- 381
- Database :
- OpenAIRE
- Journal :
- Cell and Tissue Research
- Accession number :
- edsair.doi.dedup.....414a71b56d3e29506cb95aa9be0c4cec