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Does butein affect adipogenesis?
- Source :
- Adipocyte, Adipocyte, Vol 8, Iss 1, Pp 209-222 (2019)
- Publication Year :
- 2019
- Publisher :
- TAYLOR & FRANCIS INC, 2019.
-
Abstract
- Butein is a plant flavonoid chalcone, with presumed anti-adipogenic properties. It was reported to impair preadipocyte differentiation, limit adipose tissue (AT) development and enhance white AT browning in rodents. In this study, we investigated the hypothesis that these effects of butein may occur via reduction of ADAMTS5 (A Disintegrin And Metalloproteinase with ThromboSpondin motifs 5) expression. Murine 3T3-L1 or 3T3-F442A preadipocytes were differentiated into mature adipocytes in the presence of butein or vehicle. At regular time intervals RNA was collected for gene expression studies. Male hemizygous mice for Tg(Ucp1-luc2,-tdTomato)1Kajim (ThermoMouse) were exposed to butein or vehicle, after which ATs were analyzed for Adamts5 and uncoupling protein-1 (Ucp-1) mRNA level changes. During preadipocyte differentiation, butein (25 - 50 mM) did not affect Adamts5 or Ucp-1 expression. Oil Red O analysis and monitoring of differentiation markers failed to demonstrate effects of butein on the differentiation extent. Furthermore, butein administration to the ThermoMouse (10 or 20 mg/kg, 4 days) or to the C57BL6/Rj mice (20 mg/kg, 4 weeks) did not enhance Adamts5 or Ucp-1 expression. Thus, we could not demonstrate marked effects of butein on the preadipocyte differentiation extent or AT development and browning, nor on Adamts5 or Ucp-1 gene expression during these processes. ispartof: ADIPOCYTE vol:8 issue:1 pages:209-222 ispartof: location:United States status: published
- Subjects :
- Male
EXPRESSION
Chalcone
Histology
WHITE ADIPOSE-TISSUE
Anti-Inflammatory Agents
INHIBITION
Adipose tissue
Butein
lcsh:Diseases of the endocrine glands. Clinical endocrinology
lcsh:Physiology
adipogenesis
Andrology
PATHWAY
chemistry.chemical_compound
Mice
Endocrinology & Metabolism
Chalcones
Gene expression
Adipocytes
Oil Red O
Animals
lcsh:QH573-671
Cells, Cultured
Uncoupling Protein 1
adipocyte differentiation
browning
Thrombospondin
Metalloproteinase
lcsh:RC648-665
Adipogenesis
Science & Technology
lcsh:QP1-981
lcsh:Cytology
INDUCTION
INFLAMMATORY RESPONSE
Cell Biology
3T3 Cells
Mice, Inbred C57BL
chemistry
ADAMTS5
CELLS
ADAMTS5 Protein
Life Sciences & Biomedicine
Research Paper
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Adipocyte, Adipocyte, Vol 8, Iss 1, Pp 209-222 (2019)
- Accession number :
- edsair.doi.dedup.....415695b02d7f1f308dc98d908712073d