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In vitro and in vivo cysticidal activity of extracts and isolated flavanone from the bark of Prunus serotina: A bio-guided study
- Source :
- Acta tropica. 170
- Publication Year :
- 2016
-
Abstract
- Currently, neurocysticercosis treatment involves two drugs: albendazole and praziquantel; however, their efficacy is suboptimal and new cysticidal drugs are needed. The present paper reports the cysticidal activity of extracts of the bark from Prunus serotina against Taenia crassiceps cysts and the isolation and identification of the main components of the most active extract. Results showed that all extracts displayed in vitro cysticidal activity (EC50=17.9-88.5μg/mL), being the methanolic the most active and selective. Also, methanolic extract exhibited in vivo efficacy at 300mg/kg which was similar to that obtained with albendazole. Bio-guided fractionation of methanolic extract led the isolation of 2,3-dihydro-5,7-dihydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one (naringenin, NGN), 3,4,5-trimethoxybenzoic acid and 1,3,5-trimethoxybenzene. NGN exhibited in vitro activity, in a time-concentration-dependent manner (EC50=89.3μM]. Furthermore, NGN at a dose of 376.1μmol/kg displayed similar in vivo efficacy than those obtained with albendazole at 188.4μmol/kg. NGN also caused a high level of damage in all parasite tissue in a similar manner than that observed with the methanolic extract. This study represents the first report of the cysticidal properties of the bark of P. serotina. NGN was identified as the main active compound of this specie and other studies are required to explore the potential of this flavanone as cysticidal agent.
- Subjects :
- 0301 basic medicine
Naringenin
Veterinary (miscellaneous)
030106 microbiology
Prunus avium
Albendazole
03 medical and health sciences
chemistry.chemical_compound
Mice
In vivo
medicine
Animals
EC50
Taenia crassiceps
Anthelmintics
Mice, Inbred BALB C
biology
Traditional medicine
Taenia
Cysticercosis
Plant Extracts
biology.organism_classification
Praziquantel
030104 developmental biology
Infectious Diseases
chemistry
Biochemistry
Insect Science
visual_art
Flavanones
visual_art.visual_art_medium
Parasitology
Bark
Female
Flavanone
medicine.drug
Subjects
Details
- ISSN :
- 18736254
- Volume :
- 170
- Database :
- OpenAIRE
- Journal :
- Acta tropica
- Accession number :
- edsair.doi.dedup.....4163c04e0f4c2b92c2e24d679ed49a09