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LC-MS/MS method for simultaneous determination of flavonoids and physalins in rat plasma: Application to pharmacokinetic study after oral administration of Physalis alkekengi var. franchetii (Chinese lantern) extract
- Source :
- Biomedical chromatography : BMC. 31(10)
- Publication Year :
- 2017
-
Abstract
- A rapid and sensitive liquid chromatography with tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of luteolin, luteolin-7-O-β-D-glucopyranoside, physalin A, physalin D and physalin L in rat plasma. Scutellarein and dexamethasone were used as the internal standards (IS). Plasma samples were prepared by liquid-liquid extraction with ethyl acetate. The five constituents were separated on an Acquity UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm). A gradient elution procedure was used with acetonitrile (A)-0.1% aqueous formic acid (B). Mass spectrometric detection was performed in negative ion multiple reaction monitoring mode with an electrospray ionization (ESI) source. This method showed good linearity (r2 > 0.997) over a concentration range of 2.0-500 ng/mL with a lower limit of quantification of 2.0 ng/mL for all five compounds. The inter- and intra-day accuracy ranged from 91.7 to 104%, and precisions (RSD) were 85%. This validated method was successfully applied for the first time to the pharmacokinetic study of five ingredients after oral administration of 70% ethanol extract of Chinese lantern in rats.
- Subjects :
- 0301 basic medicine
Male
Physalis
Formic acid
Electrospray ionization
Clinical Biochemistry
Ethyl acetate
Administration, Oral
Tandem mass spectrometry
01 natural sciences
Biochemistry
High-performance liquid chromatography
Analytical Chemistry
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
Drug Stability
Limit of Detection
Tandem Mass Spectrometry
Drug Discovery
Animals
Secosteroids
Molecular Biology
Pharmacology
Flavonoids
Chromatography
Scutellarein
Plant Extracts
010401 analytical chemistry
Selected reaction monitoring
Reproducibility of Results
General Medicine
0104 chemical sciences
Rats
030104 developmental biology
chemistry
Linear Models
Physalin
Chromatography, Liquid
Subjects
Details
- ISSN :
- 10990801
- Volume :
- 31
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Biomedical chromatography : BMC
- Accession number :
- edsair.doi.dedup.....418bf9ab867df33dec575a930cbea1bf