Back to Search
Start Over
Korean Scutellaria baicalensis Georgi flavonoid extract induces mitochondrially mediated apoptosis in human gastric cancer AGS cells
- Source :
- Oncology Letters
- Publication Year :
- 2017
- Publisher :
- Spandidos Publications, 2017.
-
Abstract
- Korean Scutellaria baicalensis Georgi has been widely used in Korean folk medicines for its range of medicinal benefits, including its anticancer effect. The aim of the present study was to investigate the underlying molecular mechanism of action of a flavonoid extract from Korean Scutellaria baicalensis Georgi (FSB) on AGS human gastric cancer cells (gastric adenocarcinoma) in which FSB exhibits an anticancer effect. Treatment of AGS cells with FSB significantly inhibited cell viability in a concentration-dependent manner. Furthermore, FSB significantly increased the proportion of cells in sub-G1 phase, and Annexin V and Hoechst 33258 fluorescent staining confirmed the apoptotic cell death. Furthermore, western blotting results identified that treatment of AGS cells with FSB significantly downregulated the expression of caspase family members, namely procaspases 3 and 9, and poly(ADP-ribose) polymerase (PARP), and subsequently upregulated cleaved caspase 3 and cleaved PARP. It was observed that FSB treatment significantly decreased the mitochondrial membrane potential of AGS cells. In addition, the ratio of the mitochondrion-associated proteins B cell lymphoma 2-associated X protein and B cell lymphoma extra large was upregulated. The results of the present study provide novel insight into the underlying molecular mechanism of the anticancer effects of FSB on AGS human gastric cancer cells and indicate that FSB may be an alternative chemotherapeutic agent for the treatment of gastric cancer.
- Subjects :
- 0301 basic medicine
Cancer Research
Cell
Caspase 3
03 medical and health sciences
0302 clinical medicine
medicine
Viability assay
Caspase
Korean Scutellaria baicalensis Georgi
biology
gastric cancer
apoptosis
Articles
Cell cycle
biology.organism_classification
Molecular biology
B cell lymphoma 2-associated X protein/B cell lymphoma extra large ratio
030104 developmental biology
medicine.anatomical_structure
Oncology
Apoptosis
030220 oncology & carcinogenesis
flavonoids
Cancer cell
biology.protein
Scutellaria baicalensis
Subjects
Details
- ISSN :
- 17921082 and 17921074
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Oncology Letters
- Accession number :
- edsair.doi.dedup.....41c0f5b47b6524b56fd10165894ed82b