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Cell-Wall Remodeling by the Zinc-Protease AmpDh3 from Pseudomonas aeruginosa

Cell-Wall Remodeling by the Zinc-Protease AmpDh3 from Pseudomonas aeruginosa

Authors :
Juan A. Hermoso
Mijoon Lee
Shahriar Mobashery
Cecilia Artola-Recolons
Dusan Hesek
Edward Spink
Elena Lastochkin
Weilie Zhang
Siseth Martínez-Caballero
Bill Boggess
César Carrasco-López
Lance M. Hellman
Source :
Digital.CSIC. Repositorio Institucional del CSIC, instname
Publication Year :
2013
Publisher :
American Chemical Society (ACS), 2013.

Abstract

Bacterial cell wall is a polymer of considerable complexity that is in constant equilibrium between synthesis and recycling. AmpDh3 is a periplasmic zinc protease of Pseudomonas aeruginosa, which is intimately involved in cell-wall remodeling. We document the hydrolytic reactions that this enzyme performs on the cell wall. The process removes the peptide stems from the peptidoglycan, the major constituent of the cell wall. We document that the majority of the reactions of this enzyme takes place on the polymeric insoluble portion of the cell wall, as opposed to the fraction that is released from it. We show that AmpDh3 is tetrameric both in crystals and in solution. Based on the X-ray structures of the enzyme in complex with two synthetic cell-wall-based ligands, we present for the first time a model for a multivalent anchoring of AmpDh3 onto the cell wall, which lends itself to its processive remodeling. © 2013 American Chemical Society.

Details

ISSN :
15205126 and 00027863
Volume :
135
Database :
OpenAIRE
Journal :
Journal of the American Chemical Society
Accession number :
edsair.doi.dedup.....41f9eeeba1b6082b1dfd19033f476d14
Full Text :
https://doi.org/10.1021/ja407445x