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Rapidly Measuring Scattered Polarization Parameters of the Individual Suspended Particle with Continuously Large Angular Range

Authors :
Yan Chen
Hongjian Wang
Ran Liao
Hening Li
Yihao Wang
Hu Zhou
Jiajin Li
Tongyu Huang
Xu Zhang
Hui Ma
Source :
Biosensors; Volume 12; Issue 5; Pages: 321
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

Suspended particles play a vital role in aquatic environments. We propose a method to rapidly measure the scattered polarization parameters of individual suspended particles with continuously large angular range (PCLAR), from 60° to 120° in one shot. A conceptual setup is built to measure PCLAR with 20 kHz; to verify the setup, 10 μm-diameter silica microspheres suspended in water, whose PCLAR are consistent with those simulated by Mie theory, are measured. PCLAR of 6 categories of particles are measured, which enables high-accuracy classification with the help of a convolutional neural network algorithm. PCLAR of different mixtures of Cyclotella stelligera and silica microspheres are measured to successfully identify particulate components. Furthermore, classification ability comparisons of different angular-selection strategies show that PCLAR enables the best classification beyond the single angle, discrete angles and small-ranged angles. Simulated PCLAR of particles with different size, refractive index, and structure show explicit discriminations between them. Inversely, the measured PCLAR are able to estimate the effective size and refractive index of individual Cyclotella cells. Results demonstrate the method’s power, which intrinsically takes the advantage of the optical polarization and the angular coverage. Future prototypes based on this concept would be a promising biosensor for particles in environmental monitoring.

Details

ISSN :
20796374
Volume :
12
Database :
OpenAIRE
Journal :
Biosensors
Accession number :
edsair.doi.dedup.....41fc420bca89239808eaf069c2896b52
Full Text :
https://doi.org/10.3390/bios12050321