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Polymorphism of rotavirus genotype G1 in Brazil: in silico analysis of variant strains circulating in Rio de Janeiro from 1996 to 2004
- Source :
- Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 12(7)
- Publication Year :
- 2011
-
Abstract
- The polymorphism of species A rotavirus genotype G1 strains (RVA-G1) circulating in Rio de Janeiro between 1996 and 2004 was evaluated. The VP7 encoding gene of 36 G1 isolates was sequenced and compared to references strains. The deduced amino acid sequences were used as basis for in silico analysis of the VP7 protein. We observed the circulation of two major G1 lineages and five sublineages during the studied period. Comparison between the VP7 trimeric structures of a rotavirus vaccine strain and Brazilian G1 strains showed mutations at amino acid residues located at the calcium binding site and at several neutralizing antibody recognition sites. Although the rotavirus vaccine program has clearly been successful in Brazil, these results suggest the possibility of the emergence of G1 strains that could evade the immune response elicited by a RVA vaccine and cause a vaccine breakthrough. Consequently, continuous monitoring of rotavirus intragenotypes diversity is critical to understand how it could affect vaccine effectiveness.
- Subjects :
- Microbiology (medical)
Diarrhea
Models, Molecular
Rotavirus
Genotyping
viruses
In silico
Molecular Sequence Data
medicine.disease_cause
Microbiology
Mass Vaccination
Rotavirus Infections
Evolution, Molecular
fluids and secretions
Polymorphism (computer science)
Reference Values
Genotype
Genetics
medicine
Humans
Computer Simulation
Amino Acid Sequence
Polymorphism
Neutralizing antibody
Protein Structure, Quaternary
Molecular Biology
Gene
Antigens, Viral
Ecology, Evolution, Behavior and Systematics
Phylogeny
Polymorphism, Genetic
biology
Models, Genetic
Strain (biology)
virus diseases
Sequence Analysis, DNA
Rotavirus vaccine
Virology
Protein Structure, Tertiary
Infectious Diseases
Child, Preschool
biology.protein
Capsid Proteins
Vaccine
Brazil
Subjects
Details
- ISSN :
- 15677257
- Volume :
- 12
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
- Accession number :
- edsair.doi.dedup.....42020b49c767dea9109723616a7789d4