Effect of Aromatase Inhibitor ,Sage And Flaxseed on Spermatogenesis In Rat

The study was designed to investigate estrogen effect on testis, accessory sex glands and sex hormones in male rats from weaning age at 21 days by using aromatase inhibitor letrozole ( 1 mg ̷ kg b. w. ) orally till 90 days of age and investigate Phytoestrogens effect ( sage extract Salvia Officinalis ) (1000 mg ̷ kg b.w.) orally and flaxseed (25 gm ̷ 100 gm diet ) with diet till 90 days of age . Study groups include : Group one control. Group two treated with letrozole and caused a significant decrease in body and testis weights, sperm number (zero sperms), serum estrogen hormone and luteinizing hormone concentration, and significant increase in serum follicule stimulating hormone concentration. Group three treated with flaxseed and caused significant decrease in body and prostate weight, live sperm percentage, sperms number and significant increase in the dead sperm percentage and serum lutinizing hormone concentration. Group four treated with sage extract and caused significant decrease in the body and prostate weight, sperms number. Group five Treated with letrozole and flaxseed caused significant decrease in seminal vesicle weight, serum testosterone and follicular stimulating hormone concentration and significant increase in the sperms number, live sperm percentage. Sixth group treated with letrozole and sage extract and caused significant increase in sperms number and life sperm percentage, serum estrogen hormone concentration and significant decrease in serum testosterone hormone concentration. It was concluded that inhibition and reduction of estrogen hormone concentration in male rats by treatment with aromatase inhibitor letrozole produced negative effects by decreasing the body and testes weight, live sperm percentage, sperms count, concentration of estrogen hormone while administration of flaxseed and sage extract produced positive effects in reproductive efficiency through the increase in the percentage of live sperm, estrogen and luteinizing hormone concentrations and spermatogenesis in adult rats. We concluded from the study the important role of estrogen hormone in reproductive function of male rats which cleared by the negative effects that resulted from lowering estrogen hormone level by using aromatase inhibitor letrozole and the administration of flaxseed and sage extract improved some effects produced by estrogen hormone deficiency.