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Host Restriction Factors APOBEC3G/3F and Other Interferon-Related Gene Expressions Affect Early HIV-1 Infection in Northern Pig-Tailed Macaque (Macaca leonina)
- Source :
- Frontiers in Immunology, Frontiers in Immunology, Vol 9 (2018)
- Publication Year :
- 2018
- Publisher :
- Frontiers Media S.A., 2018.
-
Abstract
- The northern pig-tailed macaques (NPMs) lack TRIM5α, an antiviral restriction factor, and instead have TRIM5-CypA. In our previous study, we demonstrated that HIV-1NL4−3 successfully infected NPMs and formed a long-term viral reservoir in vivo. However, the HIV-1-infected NPMs showed relatively high viremia in the first 6 weeks of infection, which declined thereafter suggesting that HIV-1 NL4−3 infection in these animals was only partly permissive. To optimize HIV-1 infection in NPMs therefore, we generated HIV-1NL4−R3A and stHIV-1sv, and infected NPMs with these viruses. HIV-1NL4−R3A and stHIV-1sv can replicate persistently in NPMs during 41 weeks of acute infection stage. Compared to the HIV-1NL4−R3A, stHIV-1sv showed a notably higher level of replication, and the NPMs infected with the latter induced a more robust neutralizing antibody but a weaker cellular immune response. In addition, IFN-I signaling was significantly up-regulated with the viral replication, and was higher in the stHIV-1sv infected macaques. Consequently, the sequences of pro-viral env showed fewer G-A hyper-mutations in stHIV-1sv, suggesting that vif gene of SIV could antagonize the antiviral effects of APOBEC3 proteins in NPMs. Taken together, NPMs infected with HIV-1NL4−R3A and stHIV-1sv show distinct virological and immunological features. Furthermore, interferon-related gene expression might play a role in controlling primary HIV-1NL4−R3A and stHIV-1sv replication in NPMs. This result suggests NPM is a potential HIV/AIDS animal model.
- Subjects :
- 0301 basic medicine
Male
Simian Acquired Immunodeficiency Syndrome
Gene Expression
HIV Infections
APOBEC-3G Deaminase
Virus Replication
stHIV-1sv
Antiviral Restriction Factors
Tripartite Motif Proteins
Interferon
IFN-I signaling
Gene expression
Immunology and Allergy
Neutralizing antibody
APOBEC3G
Original Research
virus diseases
APOBEC3
HIV-1NL4-R3A
Interferon Type I
Female
Simian Immunodeficiency Virus
medicine.drug
lcsh:Immunologic diseases. Allergy
Ubiquitin-Protein Ligases
030106 microbiology
Immunology
Viremia
Biology
03 medical and health sciences
Immune system
medicine
Animals
Humans
neutralizing antibodies
Gene
Genes, vif
medicine.disease
northern pig-tailed macaques
Virology
Disease Models, Animal
030104 developmental biology
Viral replication
Mutation
biology.protein
HIV-1
Macaca
lcsh:RC581-607
Carrier Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 16643224
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Frontiers in Immunology
- Accession number :
- edsair.doi.dedup.....42305501a773500f1004f919eefad67b