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Selective amplification of mouse mammary tumor virus in mammary tumors of GR mice

Authors :
J. P. Puma
Thomas G. Fanning
Robert D. Cardiff
Source :
Journal of Virology. 36:109-114
Publication Year :
1980
Publisher :
American Society for Microbiology, 1980.

Abstract

DNAs extracted from the mammary tumors of GR mice were analyzed for mouse mammary tumor virus proviral sequences by the restriction enzyme-Southern blot procedure. The tumor DNAs contain more proviral copies of mouse mammary tumor virus than DNA from a nonmalignant tissue. The degree of proviral amplification is small (ca. one to five additional copies) and appears to be variable from tumor to tumor. The restriction patterns of the amplified proviral sequences suggest a clonal origin for the tumor mass. In addition, the restriction patterns observed after digestion with the enzymes BglII and SacI indicate that only one of the proviruses endogenous to GR mice is amplified. The amplified provirus found in GR mammary tumors is identical to the provirus that is missing in GR-Mtv-2- mice, a congenic line exhibiting a low mammary tumor incidence.

Details

ISSN :
10985514 and 0022538X
Volume :
36
Database :
OpenAIRE
Journal :
Journal of Virology
Accession number :
edsair.doi.dedup.....4230f07ad5083849b1643e41f97ec112