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Laparoscopic early pregnancy diagnosis in the laboratory rat
Laparoscopic early pregnancy diagnosis in the laboratory rat
- Source :
- Laboratory Animals. 52:265-270
- Publication Year :
- 2017
- Publisher :
- SAGE Publications, 2017.
-
Abstract
- Timely and accurate diagnosis of pregnancy in the laboratory rat is an invaluable endpoint in embryological and reproductive toxicology studies. The purpose of the present study was to assess the feasibility, sensitivity, accuracy and safety of laparoscopic examination of the uterus for detecting pregnancy at an early stage, and defining the number of fetuses in surviving rat models. Female Wistar rats were subjected to a two-port laparoscopic examination of their uterus from day 6 to day 10 post-coitum (pc). Mean recovery time from anesthesia was 148 ± 66 s. A postoperative 4.0 ± 1.8% body weight loss was restored in 3.9 ± 1.3 days. Uterine bulges suggestive of underlying embryonic vesicles could be detected by day 7 pc with a 0% false-negative diagnosis. Comparison of the number of laparoscopically defined uterine bulges (fetal counts) with litter size revealed a 0% underestimation rate of fetal counts, and a 42.9–87.5% overestimation rate from day 7 to day 10 pc. In conclusion, the present study introduced the laparoscopic examination of the uterus and showed it to be a feasible, reliable and safe method of early pregnancy diagnosis in the laboratory rat by day 7 pc.
- Subjects :
- 0301 basic medicine
Litter (animal)
medicine.medical_specialty
Uterus
Early pregnancy factor
Sensitivity and Specificity
03 medical and health sciences
0302 clinical medicine
Pregnancy
medicine
Animals
Rats, Wistar
Stage (cooking)
Laparoscopy
Gynecology
Fetus
General Veterinary
medicine.diagnostic_test
biology
business.industry
medicine.disease
Rats
Surgery
Laboratory rat
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
biology.protein
Pregnancy, Animal
Female
Animal Science and Zoology
business
Subjects
Details
- ISSN :
- 17581117 and 00236772
- Volume :
- 52
- Database :
- OpenAIRE
- Journal :
- Laboratory Animals
- Accession number :
- edsair.doi.dedup.....426e625d44b9b0fe86850cb487d92557
- Full Text :
- https://doi.org/10.1177/0023677217723933