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Folding Paper-Based Aptasensor Platform Coated with Novel Nanoassemblies for Instant and Highly Sensitive Detection of 17β-Estradiol
- Source :
- ACS Sensors. 4:3186-3194
- Publication Year :
- 2019
- Publisher :
- American Chemical Society (ACS), 2019.
-
Abstract
- Owing to its critical role in the development of female reproductive tissues and as a clinical biomarker, there is an urgent need to develop a rapid and cost-effective method to sensitively detect 17β-estradiol (E2). In this work, a folding aptasensor platform with microfluidic channels for the label-free electrochemical detection of E2 is described. The platform, designed with a delicate folding structure, integrating filter holes, microfluidic channels, reaction chambers, and a three-electrode system, is extremely easy to use. To increase the detection sensitivity and immobilize the aptamer, we synthesized a novel nanoassembly consisting of amine-functionalized single-walled carbon nanotube/new methylene blue/gold nanoparticles (AuNPs) and modified the working electrode with this nanoassembly. The calibration curve obtained from the experimental results exhibited a linear range between 10 pg mL-1 and 500 ng mL-1 (R2 = 0.993), and a detection limit of 5 pg mL-1 was achieved (S/N = 3). Furthermore, experiments to detect E2 in clinical serum were conducted, and the results were highly similar to those obtained using a large electrochemical luminescence apparatus. By integrating multiple functional components, adopting novel nanoassemblies, and using a folding structure, this paper-based platform not only has great potential as a simple and convenient integrated device for point-of-care testing of E2, but also as a portable, low-cost, and highly sensitive aptasensor platform capable of detecting many diagnostic biomarkers with the appropriate aptamers.
- Subjects :
- Paper
Working electrode
Materials science
Aptamer
Metal Nanoparticles
Bioengineering
Nanotechnology
Biosensing Techniques
02 engineering and technology
01 natural sciences
chemistry.chemical_compound
Limit of Detection
Lab-On-A-Chip Devices
Humans
Electrodes
Instrumentation
Fluid Flow and Transfer Processes
Detection limit
Base Sequence
Estradiol
Nanotubes, Carbon
Process Chemistry and Technology
010401 analytical chemistry
New methylene blue
Electrochemical Techniques
Folding (DSP implementation)
Aptamers, Nucleotide
Microfluidic Analytical Techniques
021001 nanoscience & nanotechnology
0104 chemical sciences
Methylene Blue
Linear range
chemistry
Point-of-Care Testing
Colloidal gold
Gold
0210 nano-technology
Luminescence
Subjects
Details
- ISSN :
- 23793694
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- ACS Sensors
- Accession number :
- edsair.doi.dedup.....428e7ea466d64aa92e68035ed20640f8