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Global mapping of glycosylation pathways in human-derived cells
- Source :
- Dev Cell
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Glycans are one of the fundamental classes of macromolecules and are involved in a broad range of biological phenomena. A large variety of glycan structures can be synthesized depending on tissue or cell types and environmental changes. Here, we developed a comprehensive glycosylation mapping tool, termed GlycoMaple, to visualize and estimate glycan structures based on gene expression. We informatically selected 950 genes involved in glycosylation and its regulation. Expression profiles of these genes were mapped onto global glycan metabolic pathways to predict glycan structures, which were confirmed using glycomic analyses. Based on the predictions of N-glycan processing, we constructed 40 knockout HEK293 cell lines and analyzed the effects of gene knockout on glycan structures. Finally, the glycan structures of 64 cell lines, 37 tissues, and primary colon tumor tissues were estimated and compared using publicly available databases. Our systematic approach can accelerate glycan analyses and engineering in mammalian cells.
- Subjects :
- Glycan
Cell type
Glycosylation
Computational biology
Article
General Biochemistry, Genetics and Molecular Biology
Glycomics
Gene Knockout Techniques
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Polysaccharides
Cell Line, Tumor
Gene expression
Humans
Molecular Biology
Gene
Gene knockout
030304 developmental biology
0303 health sciences
biology
HEK 293 cells
Reproducibility of Results
Cell Biology
carbohydrates (lipids)
HEK293 Cells
chemistry
biology.protein
Metabolic Networks and Pathways
030217 neurology & neurosurgery
Developmental Biology
Subjects
Details
- ISSN :
- 15345807
- Volume :
- 56
- Database :
- OpenAIRE
- Journal :
- Developmental Cell
- Accession number :
- edsair.doi.dedup.....4295ff2aee78c64e077e998ea0863fa8