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Tryptanthrin Protects Mice against Dextran Sulfate Sodium-Induced Colitis through Inhibition of TNF-α/NF-κB and IL-6/STAT3 Pathways
- Source :
- Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, Molecules, Vol 23, Iss 5, p 1062 (2018), Molecules; Volume 23; Issue 5; Pages: 1062
- Publication Year :
- 2018
- Publisher :
- MDPI, 2018.
-
Abstract
- Inflammatory bowel disease (IBD) is a notable health problem and may considerably affect the quality of human life. Previously, the protective roles of tryptanthrin (TRYP) against dextran sulfate sodium (DSS) induced colitis has been proved, but the concrete mechanism remained elusive. It has been suggested that TRYP could diminish the weight loss and improve the health conditions of mice with DSS induced colitis. Hematoxylin and eosin staining revealed that TRYP could improve the histopathological structure of the colon tissue. Two signaling pathways (TNF-α/NF-κBp65 and IL-6/STAT3) were investigated using immunochemistry and western blot. The detected concentrations of the two cytokines TNF-α and IL-6 showed that their levels decreased after TRYP treatment of the colitis. The protein expression level of NF-κBp65 in cytoplasm increased after TRYP treatment of the induced colitis. However, the protein level of NF-κBp65 in the nucleus decreased after administration of TRYP. The expression level of IκBα, the inhibitory protein of NF-κBp65, was tested and the results suggested that TRYP could inhibit the degradation of IκBα. The phosphorylation level of STAT3 was inhibited by TRYP and the expression level of STAT3 and p-STAT3 decreased after administration of TRYP. We conclude that TRYP improves the health condition of mice with DSS induced colitis by regulating the TNF-α/NF-κBp65 and IL-6/STAT3 signaling pathways via inhibiting the degradation of IκBα and the phosphorylation of STAT3.
- Subjects :
- 0301 basic medicine
Male
STAT3 Transcription Factor
Pharmaceutical Science
Inflammatory bowel disease
Article
Analytical Chemistry
lcsh:QD241-441
STAT3
tryptanthrin
colitis DSS
NF-κBp65
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Western blot
lcsh:Organic chemistry
Drug Discovery
medicine
Animals
Physical and Theoretical Chemistry
Colitis
biology
medicine.diagnostic_test
Interleukin-6
Tumor Necrosis Factor-alpha
Organic Chemistry
Dextran Sulfate
NF-kappa B
NF-κB
medicine.disease
Molecular biology
IκBα
030104 developmental biology
chemistry
Chemistry (miscellaneous)
030220 oncology & carcinogenesis
biology.protein
Quinazolines
Molecular Medicine
Phosphorylation
Signal transduction
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 23
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry
- Accession number :
- edsair.doi.dedup.....42aafb0870bebaf9ede1a4d0b1014ed8