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From rabbit antibody repertoires to rabbit monoclonal antibodies
- Source :
- Experimental & Molecular Medicine
- Publication Year :
- 2016
-
Abstract
- In this review, we explain why and how rabbit monoclonal antibodies have become outstanding reagents for laboratory research and increasingly for diagnostic and therapeutic applications. Starting with the unique ontogeny of rabbit B cells that affords highly distinctive antibody repertoires rich in in vivo pruned binders of high diversity, affinity and specificity, we describe the generation of rabbit monoclonal antibodies by hybridoma technology, phage display and alternative methods, along with an account of successful humanization strategies.
- Subjects :
- 0301 basic medicine
Phage display
medicine.drug_class
Clinical Biochemistry
Review
Monoclonal antibody
Antibodies, Monoclonal, Humanized
Biochemistry
03 medical and health sciences
0302 clinical medicine
Immunologic Technique
In vivo
medicine
Animals
Humans
Molecular Biology
biology
Rabbit (nuclear engineering)
Molecular biology
030104 developmental biology
030220 oncology & carcinogenesis
Monoclonal
biology.protein
Immunologic Techniques
Molecular Medicine
Hybridoma technology
Rabbits
Antibody
Subjects
Details
- ISSN :
- 20926413
- Volume :
- 49
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Experimentalmolecular medicine
- Accession number :
- edsair.doi.dedup.....42b9214c03adf6b02de3420959593a51