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Microfluidic-enabled self-organized tumor model for in vitro cytotoxicity assessment of doxorubicin
- Source :
- Biomedical microdevices. 22(4)
- Publication Year :
- 2020
-
Abstract
- The advent of microfluidic technologies has enabled a better recapitulation of in vitro tumor model with higher biological relevance over conventional monolayer assays. This work built upon a microfluidic system that supported the spontaneous aggregate formation of tumoral cells under flow-induced dynamic physical forces in a confined microchamber without additional matrix materials. Our findings indicated that fluidic streams significantly modulated the biological and architectural features of human breast adenocarcinoma cell (MCF-7), human hepatocarcinoma cell (HepG2), and human cervix adenocarcinoma cell (HeLa) with cell-type-dependent variation. The microfluidic platform was further integrated with a fluorescence detection and imaging system, allowing for non-invasive monitoring of cellular accumulation and spatial distribution of a chemotherapeutic agent, doxorubicin (DOX). The cytotoxic effects of DOX of various concentrations were determined and compared in MCF-7 cells in conventional two-dimensional (2D) static and microfluidic culture conditions. Dose-dependent response to DOX was noticed in both cultures, whereas tumor micronodules grown in microfluidic devices demonstrated significantly lower sensitivity to DOX at increased concentration. Our platform owns promising potentials as a universal modality for bridging traditional 2D cell cultures and in vivo experimentation for preclinical anticancer drug screening.
- Subjects :
- Cell
Microfluidics
Biomedical Engineering
Antineoplastic Agents
02 engineering and technology
01 natural sciences
HeLa
In vivo
Lab-On-A-Chip Devices
medicine
Cytotoxic T cell
Humans
Doxorubicin
Molecular Biology
biology
Chemistry
010401 analytical chemistry
021001 nanoscience & nanotechnology
biology.organism_classification
In vitro
0104 chemical sciences
medicine.anatomical_structure
Cell culture
Biophysics
MCF-7 Cells
Drug Screening Assays, Antitumor
0210 nano-technology
medicine.drug
HeLa Cells
Subjects
Details
- ISSN :
- 15728781
- Volume :
- 22
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Biomedical microdevices
- Accession number :
- edsair.doi.dedup.....42bfbf1e80987cca1bc8d016b16e0876