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A hepatitis B virus pre-S-retinoic acid receptor beta chimera transforms erythrocytic progenitor cells in vitro
- Source :
- Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences of the United States of America, 1993, 90, pp.89-93. ⟨10.1073/pnas.90.1.89⟩, Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 1993, 90, pp.89-93. ⟨10.1073/pnas.90.1.89⟩
- Publication Year :
- 1993
- Publisher :
- HAL CCSD, 1993.
-
Abstract
- International audience; In this report, we investigated the transforming properties of retinoic acid receptor beta (RAR beta). The v-erbA protein, which is the viral oncogenic homologue of the thyroid hormone receptor, was replaced by either the complete RAR beta (beta R) or a hepatitis B virus pre-S-RAR beta (H beta R) hybrid product in an avian erythroblastosis virus-based vector. In chicken hematopoietic cells, the H beta R protein was able to transform erythroid progenitor cells, whereas no such transformation was observed with the wild-type beta R protein. Moreover, the fully transformed phenotype was observed even in the absence of v-erbB, and H beta R-transformed erythroid cells grew independently of growth factors and transforming growth factor alpha. The analysis of erythrocytic-specific proteins revealed that the transformed cells were blocked at the colony-forming unit-erythroid stage and that the expression of the carbonic anhydrase II gene, a gene normally regulated by thyroid hormones, was repressed by the H beta R protein. Finally, hepatocarcinomas rapidly developed in some chickens infected in ovo with viruses encoding either the normal or the hybrid H beta R, suggesting that an inappropriate expression of the RAR beta gene may represent an important event in oncogenesis.
- Subjects :
- TGF alpha
Erythrocytes
Receptors, Retinoic Acid
[SDV]Life Sciences [q-bio]
Restriction Mapping
Retroviridae Proteins, Oncogenic
Retinoic acid
Retinoic acid receptor beta
Chick Embryo
chemistry.chemical_compound
0302 clinical medicine
Liver Neoplasms, Experimental
Viral Envelope Proteins
Carbonic Anhydrases
Cell Line, Transformed
0303 health sciences
Multidisciplinary
Liver Neoplasms
Oncogene Proteins v-erbA
Protein-Tyrosine Kinases
3. Good health
Isoenzymes
Cell Transformation, Neoplastic
030220 oncology & carcinogenesis
Cell Division
Research Article
Hepatitis B virus
Recombinant Fusion Proteins
Molecular Sequence Data
Bone Marrow Cells
Biology
03 medical and health sciences
Animals
Humans
Amino Acid Sequence
Interleukin 12 receptor, beta 1 subunit
030304 developmental biology
Thyroid hormone receptor
Base Sequence
Oncogenes
TGF beta receptor 2
Endoglin
Hematopoietic Stem Cells
Virology
Molecular biology
chemistry
Enzyme Repression
Carrier Proteins
Chickens
Subjects
Details
- Language :
- English
- ISSN :
- 00278424 and 10916490
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences of the United States of America, 1993, 90, pp.89-93. ⟨10.1073/pnas.90.1.89⟩, Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 1993, 90, pp.89-93. ⟨10.1073/pnas.90.1.89⟩
- Accession number :
- edsair.doi.dedup.....42e773c6f778ce2772b0f50506f038f0
- Full Text :
- https://doi.org/10.1073/pnas.90.1.89⟩