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Combinatorial Analysis of Transcription Factor Partners Reveals Recruitment of c-MYC to Estrogen Receptor-α Responsive Promoters
- Source :
- Molecular Cell. 21:393-404
- Publication Year :
- 2006
- Publisher :
- Elsevier BV, 2006.
-
Abstract
- In breast cancer and normal estrogen target tissues, estrogen receptor-alpha (ERalpha) signaling results in the establishment of spatiotemporal patterns of gene expression. Whereas primary target gene regulation by ERalpha involves recruitment of coregulatory proteins, coactivators, or corepressors, activation of these downstream promoters by receptor signaling may also involve partnership of ERalpha with other transcription factors. By using an integrated, genome-wide approach that involves ChIP-chip and computational modeling, we uncovered 13 ERalpha-responsive promoters containing both ERalpha and c-MYC binding elements located within close proximity (13-214 bp) to each other. Estrogen stimulation enhanced the c-MYC-ERalpha interaction and facilitated the association of ERalpha, c-MYC, and the coactivator TRRAP with these estrogen-responsive promoters, resulting in chromatin remodeling and increased transcription. These results suggest that ERalpha and c-MYC physically interact to stabilize the ERalpha-coactivator complex, thereby permitting other signal transduction pathways to fine-tune estrogen-mediated signaling networks.
- Subjects :
- Transcription, Genetic
Genes, myc
Estrogen receptor
Biology
Chromatin remodeling
Cell Line
03 medical and health sciences
0302 clinical medicine
Coactivator
Animals
Humans
Computer Simulation
Promoter Regions, Genetic
Molecular Biology
Transcription factor
Estrogen receptor beta
Oligonucleotide Array Sequence Analysis
030304 developmental biology
0303 health sciences
Gene Expression Profiling
Estrogen Receptor alpha
Estrogens
Promoter
Cell Biology
Chromatin
Gene Expression Regulation
030220 oncology & carcinogenesis
Cancer research
RNA Interference
Signal transduction
Estrogen receptor alpha
Transcription Factors
Subjects
Details
- ISSN :
- 10972765
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Molecular Cell
- Accession number :
- edsair.doi.dedup.....42e7fb900f304b19d2476a05911a6c97