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Structural basis for oligomerization of auxin transcriptional regulators
- Source :
- Nature Communications, Nature Communications, Nature Publishing Group, 2014, 5, pp.3617. ⟨10.1038/ncomms4617⟩, Nature Communications (5), . (2014), Nature Communications, 2014, 5, pp.3617. ⟨10.1038/ncomms4617⟩
- Publication Year :
- 2014
- Publisher :
- HAL CCSD, 2014.
-
Abstract
- Supplementary Information Supplementary Figures 1-3 and Supplementary Tables 1-2; International audience; The plant hormone auxin is a key morphogenetic regulator acting from embryogenesis onwards. Transcriptional events in response to auxin are mediated by the auxin response factor (ARF) transcription factors and the Aux/IAA (IAA) transcriptional repressors. At low auxin concentrations, IAA repressors associate with ARF proteins and recruit corepressors that prevent auxin-induced gene expression. At higher auxin concentrations, IAAs are degraded and ARFs become free to regulate auxin-responsive genes. The interaction between ARFs and IAAs is thus central to auxin signalling and occurs through the highly conserved domain III/IV present in both types of proteins. Here, we report the crystal structure of ARF5 domain III/IV and reveal the molecular determinants of ARF-IAA interactions. We further provide evidence that ARFs have the potential to oligomerize, a property that could be important for gene regulation in response to auxin.
- Subjects :
- 0106 biological sciences
Protein domain
Arabidopsis
General Physics and Astronomy
Repressor
plant
Biology
phytohormone
Crystallography, X-Ray
01 natural sciences
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Plant Growth Regulators
Gene Expression Regulation, Plant
Transcription (biology)
Auxin
Gene expression
Morphogenesis
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Protein Interaction Domains and Motifs
heterocyclic compounds
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
régulateur de transcription
Transcription factor
transcription factor
030304 developmental biology
Regulation of gene expression
chemistry.chemical_classification
0303 health sciences
Multidisciplinary
Indoleacetic Acids
auxine
Arabidopsis Proteins
fungi
food and beverages
General Chemistry
biology.organism_classification
Protein Structure, Tertiary
Cell biology
DNA-Binding Proteins
chemistry
Biochemistry
Plant hormone
auxin
gene regulation
Signal Transduction
Transcription Factors
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Database :
- OpenAIRE
- Journal :
- Nature Communications, Nature Communications, Nature Publishing Group, 2014, 5, pp.3617. ⟨10.1038/ncomms4617⟩, Nature Communications (5), . (2014), Nature Communications, 2014, 5, pp.3617. ⟨10.1038/ncomms4617⟩
- Accession number :
- edsair.doi.dedup.....4321764c696990134f8bbeb758ae485c