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Healing of bone defects by induced pluripotent stem cell-derived bone marrow mesenchymal stem cells seeded on hydroxyapatite-zirconia
- Source :
- Ann Transl Med
- Publication Year :
- 2021
-
Abstract
- BACKGROUND: Induced pluripotent stem cells (iPSCs) can generate bone marrow mesenchymal stem cells (BMSCs) as seed cells for tissue-engineered bone to repair bone defects. In this study, we investigated the effects of hydroxyapatite-zirconia (HA/ZrO(2)) composites combined with iPSC-derived BMSCs as a bone substitute on repairing skull defects in rats. METHODS: Human urinary cells isolated from a healthy donor were reprogramed into iPSCs and then induced into BMSCs. Immunocytochemistry (IHC) and reverse transcription-polymerase chain reaction (RT-PCR) were used to examine the characteristics of the induced MSCs. The iPSC-derived BMSCs were cultured on HA/ZrO(2) composites, and cytocompatibility of the composite was analyzed by cell counting kit-8 (CCK-8) assays, RT-PCR, and scanning electron microscopy. Then, HA/ZrO(2) combined with iPSC-derived expanded potential stem cells (EpSCs) were transplanted onto skull defects of rats. The effects of this composite on bone repair were evaluated by IHC. RESULTS: The results showed that MSCs induced from iPSCs displayed the phenotypes and property of normal BMSCs. After seeding on the HA/ZrO(2), iPSC-derived BMSCs had the ability to proliferate and differentiate into osteoblasts. After transplantation, iPSC-derived BMSCs on HA/ZrO(2) promoted construction of bone on rat skulls. CONCLUSIONS: These results indicated that transplantation of a HA/ZrO(2) combined with iPS-derived BMSCs is feasible to reconstruct bones and may be a substantial reference for iPSC-based therapy for bone defects.
- Subjects :
- stomatognathic system
Original Article
General Medicine
Subjects
Details
- ISSN :
- 23055839
- Volume :
- 9
- Issue :
- 23
- Database :
- OpenAIRE
- Journal :
- Annals of translational medicine
- Accession number :
- edsair.doi.dedup.....4326f3dbb1a781f97accc3c0caf1fb0d