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Of men not mice: Bactericidal/permeability-increasing protein (BPI) expressed in human macrophages acts as a phagocytic receptor and modulates entry and replication of Gram-negative bacteria
- Source :
- Frontiers in Immunology, Vol 7 (2016), Frontiers in Immunology
- Publication Year :
- 2016
- Publisher :
- Frontiers Media S.A., 2016.
-
Abstract
- Macrophages as immune cells prevent the spreading of pathogens by means of active phagocytosis and killing. We report here the presence of an anti-microbial protein, bactericidal/permeability-increasing protein (BPI) in human macrophages, which actively participates in engulfment and killing of Gram-negative pathogens. Our studies revealed increased expression of BPI in human macrophages during bacterial infection and upon stimulation with various PAMPs (Pathogen-associated molecular patterns) viz., LPS, flagellin. Further, during the course of an infection, BPI interacted with Gram- negative bacteria, resulting in enhanced phagocytosis and subsequent control of the bacterial replication. However, it was observed that bacteria which can maintain an active replicating niche (Salmonella Typhimurium) avoid the interaction with BPI during later stages of infection. On the other hand, Salmonella mutants, which cannot maintain a replicating niche, as well as Shigella flexneri, which quit the endosomal vesicle, showed interaction with BPI. These results propose an active role of BPI in Gram-negative bacterial clearance by human macrophages.
- Subjects :
- 0301 basic medicine
lcsh:Immunologic diseases. Allergy
Gram-negative bacteria
Phagocytosis
Immunology
phagocytic receptor
antimicrobial protein
Microbiology
03 medical and health sciences
0302 clinical medicine
Immune system
Shigella flexneri
Anti-microbial protein
Centre for Biosystems Science and Engineering
Gram-Negative Bacteria
Immunology and Allergy
innate immunity
Original Research
Microbiology & Cell Biology
Innate immune system
biology
Macrophage evolution
biology.organism_classification
Bactericidal/permeability-increasing protein
humanities
Bacterial niche
030104 developmental biology
030220 oncology & carcinogenesis
biology.protein
lcsh:RC581-607
Flagellin
Bacteria
Subjects
Details
- Language :
- English
- ISSN :
- 16643224
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Frontiers in Immunology
- Accession number :
- edsair.doi.dedup.....43794b3f00a1d74fa7ca75e2d472b325