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Untargeted Metabolomic Analysis of Amniotic Fluid in the Prediction of Preterm Delivery and Bronchopulmonary Dysplasia
- Source :
- PLoS ONE, Vol 11, Iss 10, p e0164211 (2016), PLoS ONE
- Publication Year :
- 2016
- Publisher :
- Public Library of Science (PLoS), 2016.
-
Abstract
- Objective Bronchopulmonary dysplasia (BPD) is a serious complication associated with preterm birth. A growing body of evidence suggests a role for prenatal factors in its pathogenesis. Metabolomics allows simultaneous characterization of low molecular weight compounds and may provide a picture of such a complex condition. The aim of this study was to evaluate whether an unbiased metabolomic analysis of amniotic fluid (AF) can be used to investigate the risk of spontaneous preterm delivery (PTD) and BPD development in the offspring. Study design We conducted an exploratory study on 32 infants born from mothers who had undergone an amniocentesis between 21 and 28 gestational weeks because of spontaneous preterm labor with intact membranes. The AF samples underwent untargeted metabolomic analysis using mass spectrometry combined with ultra-performance liquid chromatography. The data obtained were analyzed using multivariate and univariate statistical data analysis tools. Results Orthogonally Constrained Projection to Latent Structures-Discriminant Analysis (oCPLS2-DA) excluded effects on data modelling of crucial clinical variables. oCPLS2-DA was able to find unique differences in select metabolites between term (n = 11) and preterm (n = 13) deliveries (negative ionization data set: R2 = 0.47, mean AUC ROC in prediction = 0.65; positive ionization data set: R2 = 0.47, mean AUC ROC in prediction = 0.70), and between PTD followed by the development of BPD (n = 10), and PTD without BPD (n = 11) (negative data set: R2 = 0.48, mean AUC ROC in prediction = 0.73; positive data set: R2 = 0.55, mean AUC ROC in prediction = 0.71). Conclusions This study suggests that amniotic fluid metabolic profiling may be promising for identifying spontaneous preterm birth and fetuses at risk for developing BPD. These findings support the hypothesis that some prenatal metabolic dysregulations may play a key role in the pathogenesis of PTD and the development of BPD.
- Subjects :
- 0301 basic medicine
Male
Genetics and Molecular Biology (all)
Amniotic fluid
Physiology
Maternal Health
lcsh:Medicine
Medicine (all)
Biochemistry, Genetics and Molecular Biology (all)
Agricultural and Biological Sciences (all)
Biochemistry
Mass Spectrometry
Database and Informatics Methods
0302 clinical medicine
Pregnancy
Prenatal Diagnosis
Metabolites
Medicine and Health Sciences
Database Searching
lcsh:Science
Chromatography, High Pressure Liquid
Statistical Data
Bronchopulmonary Dysplasia
Multidisciplinary
medicine.diagnostic_test
Obstetrics
Gestational age
Obstetrics and Gynecology
Discriminant Analysis
Body Fluids
Premature birth
Obstetric Procedures
Area Under Curve
Physical Sciences
Amniocentesis
Metabolome
Premature Birth
Female
Anatomy
Statistics (Mathematics)
Research Article
medicine.medical_specialty
Offspring
Surgical and Invasive Medical Procedures
Gestational Age
Preterm Birth
Research and Analysis Methods
03 medical and health sciences
Diagnostic Medicine
medicine
Metabolomics
Humans
Least-Squares Analysis
Fetus
business.industry
Preterm Labor
lcsh:R
Infant, Newborn
Biology and Life Sciences
medicine.disease
Amniotic Fluid
Pregnancy Complications
030104 developmental biology
Metabolism
030228 respiratory system
Bronchopulmonary dysplasia
ROC Curve
Birth
Women's Health
lcsh:Q
business
Mathematics
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....43b120ed9da5acc95279ef529dbdd104