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Hsp16p Is Required for Thermotolerance in Nuclear mRNA Export in Fission Yeast Schizosaccharomyces pombe
- Source :
- Cell Structure and Function. 29:125-138
- Publication Year :
- 2005
- Publisher :
- Japan Society for Cell Biology, 2005.
-
Abstract
- Export of mRNA from the nucleus to the cytoplasm is one of the essential steps for eukaryotic gene expression. In the fission yeast Schizosaccharomyces pombe, heat shock stress at 42 degrees C causes block of mRNA export from the nucleus. We now report that saline and ethanol stresses also inhibit nuclear mRNA export, resulting in accumulation of bulk poly (A)+ RNA, as well as a specific mRNA, in the nucleus. Under stressed conditions, an mRNA export receptor Mex67p relocates to the nucleolus from the nuclear periphery and this relocation is closely correlated with inhibition of mRNA export by the stresses. Pretreatment of cells with a mild saline stress induced thermotolerance in mRNA export in a similar manner seen with mild heat pretreatment and protected mRNA export machinery from the subsequent severe heat shock. In contrast, mild ethanol stress could not induce the thermotolerance in mRNA export, suggesting that the stress response induced by ethanol differs from that induced by saline and heat shock stresses. In addition, we found that the Spc1p MAPK pathway is involved in induction of thermotolerance in mRNA export. Of the downstream targets for Spc1p, the Atf1p transcription factor was essential for induction of thermotolerance in mRNA export. We also found that Hsp16p, the expression of which is controlled by Atf1p, is involved in acquisition of thermotolerance in mRNA export in S. pombe.
- Subjects :
- MAPK/ERK pathway
Hot Temperature
Physiology
Nucleolus
Active Transport, Cell Nucleus
Receptors, Cytoplasmic and Nuclear
Sodium Chloride
Biology
Schizosaccharomyces
Gene expression
RNA, Messenger
Molecular Biology
Transcription factor
Heat-Shock Proteins
In Situ Hybridization, Fluorescence
Cell Nucleus
Messenger RNA
Ethanol
RNA
Cell Biology
General Medicine
biology.organism_classification
Molecular biology
Cytoplasm
Schizosaccharomyces pombe
Schizosaccharomyces pombe Proteins
Cell Nucleolus
Heat-Shock Response
Transcription Factors
Subjects
Details
- ISSN :
- 13473700 and 03867196
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Cell Structure and Function
- Accession number :
- edsair.doi.dedup.....43c0c24fa9861225304febe4fa1825df