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Extended depth of focus multiphoton microscopy via incoherent pulse splitting

Authors :
Reto Fiolka
James D. Manton
Stephan Daetwyler
Bingying Chen
Tonmoy Chakraborty
Kevin M. Dean
Source :
Biomed Opt Express
Publication Year :
2020
Publisher :
Optical Society of America, 2020.

Abstract

We present a beam splitter mask that can be easily added to a multiphoton raster scanning microscope to extend the depth of focus five-fold at a small loss in lateral resolution. The method is designed for ultrafast laser pulses or other light-sources featuring a low coherence length. In contrast to other methods of focus extension, our approach uniquely combines low complexity, high light-throughput and multicolor capability. We characterize the point spread function in a two-photon microscope and demonstrate fluorescence imaging of GFP labeled neurons in fixed brain samples as imaged with conventional and extended depth of focus two-photon microscopy.

Details

Language :
English
Database :
OpenAIRE
Journal :
Biomed Opt Express
Accession number :
edsair.doi.dedup.....43da8fb2edbc88a888a79d6daab11ff9