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Extended depth of focus multiphoton microscopy via incoherent pulse splitting
- Source :
- Biomed Opt Express
- Publication Year :
- 2020
- Publisher :
- Optical Society of America, 2020.
-
Abstract
- We present a beam splitter mask that can be easily added to a multiphoton raster scanning microscope to extend the depth of focus five-fold at a small loss in lateral resolution. The method is designed for ultrafast laser pulses or other light-sources featuring a low coherence length. In contrast to other methods of focus extension, our approach uniquely combines low complexity, high light-throughput and multicolor capability. We characterize the point spread function in a two-photon microscope and demonstrate fluorescence imaging of GFP labeled neurons in fixed brain samples as imaged with conventional and extended depth of focus two-photon microscopy.
- Subjects :
- Point spread function
0303 health sciences
Fluorescence-lifetime imaging microscopy
Depth of focus
Materials science
Microscope
business.industry
Laser
01 natural sciences
Atomic and Molecular Physics, and Optics
Article
Coherence length
law.invention
010309 optics
03 medical and health sciences
Optics
law
0103 physical sciences
Microscopy
business
Raster scan
Ultrashort pulse
Biotechnology
030304 developmental biology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Biomed Opt Express
- Accession number :
- edsair.doi.dedup.....43da8fb2edbc88a888a79d6daab11ff9