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Hypothermic Liver Machine Perfusion With EKPS-1 Solution vs Aqix RS-I Solution: In Vivo Feasibility Study in a Pig Transplantation Model
- Source :
- Transplantation proceedings. 41(2)
- Publication Year :
- 2009
-
Abstract
- Objective Hypothermic machine perfusion (HMP) is superior to simple cold storage for kidney preservation. We previous observed in a porcine liver transplantation model increased tumor necrosis factor–α (TNF-α) production eventually leading to poor recipient survival after HMP using standard kidney perfusion solution (KPS-1) compared with simple cold storage. We compared two solutions for HMP preservation of the liver: enriched KPS-1 (EKPS-1) and Aqix RS-I. Methods Pig livers were obtained after cold flushing with histidine-tryptophan-ketoglutarate solution. Subsequently, the livers were subjected to dual-vessel perfusion with two preservation solutions: EKPS-1 (n = 6) and Aqix RS-I (n = 3). After HMP preservation and transplantation, graft and recipient survival, hepatocellular damage (aspartate aminotransferase concentration), TNF-α production, and endothelial cell damage (hyaluronic acid clearance) were recorded. Results No primary graft nonfunction was observed. Recipient survival at postoperative day 3 was similar in both groups (33%). Aspartate aminotransferase concentration measured in serum samples after reperfusion was similar in both groups. After reperfusion, TNF-α concentration was higher and hyaluronic acid clearance was lower in the EKPS-1 group vs the Aqix RS-I group at 60, 120, and 180 minutes (all P Conclusion Hypothermic machine perfusion provided adequate longer term graft survival. After reperfusion, TNF-α production seems to be reduced, and endothelial cell dysfunction remains pronounced with Aqix RS-1 solution compared with EKPS-1 solution.
- Subjects :
- Pathology
medicine.medical_specialty
Swine
Organ Preservation Solutions
Cold storage
Andrology
chemistry.chemical_compound
In vivo
Hypothermia, Induced
Hyaluronic acid
medicine
Animals
Transplantation
Machine perfusion
business.industry
Portal Vein
Graft Survival
Organ Preservation
Hypothermia
Liver Transplantation
Endothelial stem cell
Perfusion
chemistry
Liver
Models, Animal
Feasibility Studies
Surgery
medicine.symptom
business
Subjects
Details
- ISSN :
- 00411345
- Volume :
- 41
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Transplantation proceedings
- Accession number :
- edsair.doi.dedup.....43e3fed7640a811a0047baf3c56b3c15