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A Predicted Mannoprotein Cmp1 Regulates Fungal Virulence in Cryptococcus neoformans
- Source :
- Pathogens, Pathogens, Vol 9, Iss 881, p 881 (2020), Volume 9, Issue 11
- Publication Year :
- 2020
- Publisher :
- MDPI, 2020.
-
Abstract
- The capsule of the fungal pathogen Cryptococcus neoformans consists of glucuronoxylomannan (GXM), glucuronoxylomannogalactan (GXMGal), and mannoproteins (MPs). MPs are a kind of glycoproteins with low content but high immunogenicity, which can stimulate the immune protection of the host. However, there is not much information about the role of mannoproteins in virulence of the human fungal pathogen C. neoformans. In this study, we reported the identification and functional analysis of a predicted mannoprotein Cmp1 that regulates fungal virulence in C. neoformans. Gene expression pattern analysis indicates that the CMP1 gene was ubiquitously expressed at all stages of cryptococcal development. Subcellular localization analysis indicated that Cmp1 was localized in the cytoplasm of cryptococcal cells. Disruption or overexpression of CMP1 results in impairing capsule formation in Cryptococcus, but it does not affect the melanin production and sensitivity under various stress conditions, nor does it affect the sexual reproduction process of Cryptococcus. Survival assay showed that the pathogenicity of the cmp1&Delta<br />mutant or the CMP1 overexpression strain was significantly attenuated in a murine inhalation model of cryptococcosis. In conclusion, our findings implied that the mannoprotein Cmp1 is required for the virulence of C. neoformans.
- Subjects :
- Microbiology (medical)
capsule
Mutant
Cryptococcus
Virulence
lcsh:Medicine
Article
Microbiology
Gentamicin protection assay
medicine
Immunology and Allergy
mannoprotein
Molecular Biology
Gene
Cryptococcus neoformans
General Immunology and Microbiology
biology
Immunogenicity
lcsh:R
Cmp1
biology.organism_classification
medicine.disease
carbohydrates (lipids)
virulence
Infectious Diseases
Cryptococcosis
Subjects
Details
- Language :
- English
- ISSN :
- 20760817
- Volume :
- 9
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Pathogens
- Accession number :
- edsair.doi.dedup.....43ed97351881a8494c80d9948a69e838