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In vivo fluorescence molecular imaging of the vascular endothelial growth factor in rats with early diabetic retinopathy
- Source :
- Biomed Opt Express
- Publication Year :
- 2021
- Publisher :
- Optica Publishing Group, 2021.
-
Abstract
- Anti-vascular endothelial growth factor (anti-VEGF) therapy is effective for reducing the severity level of diabetic retinopathy (DR). However, it is difficult to determine the in vivo spatial and temporal expression of VEGF in the DR retina at an early stage. Here, we report a quantitatively fluorescence molecular imaging and image analysis method by creating a VEGF targeted fluorescence imaging probe, which can potentially detect and predict anti-VEGF treatment response. Moreover, the ex vivo multiscale fluorescence imaging demonstrated the spatial correlation between VEGF relative expression and vascular abnormalities in two and three dimensions. It revealed that VEGF was mainly abnormally expressed at the bifurcation of the microvessels, which advances the knowledge of the DR progression by molecular fluorescence imaging. Our study has the potential to achieve early detection of DR disease, provide more insight into understanding anti-VEGF treatment, and may help stratify patients based on the molecular imaging of retinal VEGF.
- Subjects :
- Fluorescence-lifetime imaging microscopy
Pathology
medicine.medical_specialty
business.industry
Growth factor
medicine.medical_treatment
Diabetic retinopathy
medicine.disease
Article
Atomic and Molecular Physics, and Optics
Vascular endothelial growth factor
chemistry.chemical_compound
chemistry
In vivo
medicine
Molecular imaging
business
Ex vivo
Preclinical imaging
Biotechnology
Subjects
Details
- ISSN :
- 21567085
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Biomedical Optics Express
- Accession number :
- edsair.doi.dedup.....440fb9fe90e467aa3cd8a7e6d09a996c