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Schistosoma japonicum: a PCR assay for the early detection and evaluation of treatment in a rabbit model
- Source :
- Experimental parasitology. 121(2)
- Publication Year :
- 2008
-
Abstract
- A specific PCR assay for the detection of Schistosoma japonicum DNA in rabbit fecal and serum samples was developed by amplifying a 230-bp fragment from the sequence information of the clone G55A of the highly repetitive retrotransposon SjR2. The minimum amount of DNA detectable using the PCR assay was 0.8pg, and the expected PCR product was amplified when DNA equivalent of 1.1 egg from feces was used as template. In the meantime, serum anti-worm IgG was examined by ELISA. ELISA gave positive results at 4-6 weeks post-infection depending on the cercarial doses. The parasite eggs were detected in feces at 7 weeks post-infection. In contrast, S. japonicum DNA was detected in sera at first week post-infection, and it became negative at 10 weeks post-treatment, whereas the anti-worm IgG was still at high levels at 23 weeks post-treatment. These data demonstrated that the PCR assay established provides a potential tool for the early diagnosis and therapy evaluation for S. japonicum infection in humans.
- Subjects :
- clone (Java method)
Male
Immunology
Enzyme-Linked Immunosorbent Assay
Polymerase Chain Reaction
Sensitivity and Specificity
Praziquantel
Schistosoma japonicum
law.invention
chemistry.chemical_compound
Feces
Random Allocation
law
parasitic diseases
medicine
Parasite hosting
Helminths
Animals
Polymerase chain reaction
Anthelmintics
biology
General Medicine
DNA, Helminth
biology.organism_classification
Molecular biology
Disease Models, Animal
Infectious Diseases
chemistry
Schistosomiasis japonica
Parasitology
Female
Rabbits
DNA
medicine.drug
Subjects
Details
- ISSN :
- 10902449
- Volume :
- 121
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Experimental parasitology
- Accession number :
- edsair.doi.dedup.....443b9cad787f70de0bdfcbddd59fe780