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Naringin Protects Against High Glucose-Induced Human Endothelial Cell Injury Via Antioxidation and CX3CL1 Downregulation
- Source :
- Cellular Physiology and Biochemistry, Vol 42, Iss 6, Pp 2540-2551 (2017)
- Publication Year :
- 2017
- Publisher :
- S. Karger AG, 2017.
-
Abstract
- Background/Aims: The induction of endothelial injury by hyperglycemia in diabetes has been widely accepted. Naringin is a bio-flavonoid. Some studies showed that naringin alleviates diabetic complications, but the exact mechanisms by which naringin improves diabetic anomalies are not yet fully understood. The aim of this research was to study the protective effect of naringin on high glucose-induced injury of human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were cultured with or without high glucose in the absence or presence of naringin for 5 days. The expression of CX3CL1 was determined by quantitative real-time RT-PCR (qPCR) and western blot. The cellular bioenergetic analysis oxygen consumption rate (OCR) was measured with a Seahorse Bioscience XF analyzer. Results: The production of reactive oxygen species (ROS), the expression of CX3CL1 and the level of AKT phosphorylation were increased in HUVECs cultured with high glucose compared with controls. However, naringin rescued these increases in ROS production, CX3CL1 expression and AKT phosphorylation. Nitric oxide (NO) production and OCR were lower in the high glucose group, and naringin restored the changes induced by high glucose. Molecular docking results suggested that Naringin might interact with the CX3CL1 protein. Conclusion: Naringin protects HUVECs from high-glucose-induced damage through its antioxidant properties by downregulating CX3CL1 and by improving mitochondrial function.
- Subjects :
- 0301 basic medicine
Cell Survival
Physiology
Down-Regulation
Oxygen consumption rate
Pharmacology
Mitochondrion
Nitric Oxide
Protective Agents
lcsh:Physiology
Umbilical vein
CX3CL1
Nitric oxide
lcsh:Biochemistry
03 medical and health sciences
chemistry.chemical_compound
HUVEC
Oxygen Consumption
0302 clinical medicine
Downregulation and upregulation
Western blot
Catalytic Domain
Human Umbilical Vein Endothelial Cells
medicine
Humans
lcsh:QD415-436
Phosphorylation
Naringin
Cell Proliferation
chemistry.chemical_classification
Reactive oxygen species
Binding Sites
lcsh:QP1-981
medicine.diagnostic_test
Chemokine CX3CL1
ROS
Mitochondria
Molecular Docking Simulation
Endothelial stem cell
Oxidative Stress
Glucose
030104 developmental biology
chemistry
Biochemistry
030220 oncology & carcinogenesis
Flavanones
Reactive Oxygen Species
Proto-Oncogene Proteins c-akt
Subjects
Details
- ISSN :
- 14219778 and 10158987
- Volume :
- 42
- Database :
- OpenAIRE
- Journal :
- Cellular Physiology and Biochemistry
- Accession number :
- edsair.doi.dedup.....444ddeeece5dce1120caf44e08110fa3