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Idiotype vaccines produced with a non-cytopathic alphavirus self-amplifying RNA vector induce antitumor responses in a murine model of B-cell lymphoma
- Source :
- Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021), Scientific Reports
- Publication Year :
- 2021
- Publisher :
- Nature Portfolio, 2021.
-
Abstract
- A promising therapy for patients with B-cell lymphoma is based on vaccination with idiotype monoclonal antibodies (mAbs). Since idiotypes are different in each tumor, a personalized vaccine has to be produced for each patient. Expression of immunoglobulins with appropriate post-translational modifications for human use often requires the use of stable mammalian cells that can be scaled-up to reach the desired level of production. We have used a noncytopathic self-amplifying RNA vector derived from Semliki Forest virus (ncSFV) to generate BHK cell lines expressing murine follicular lymphoma-derived idiotype A20 mAb. ncSFV/BHK cell lines expressed approximately 2 mg/L/24 h of A20 mAb with proper quaternary structure and a glycosylation pattern similar to that of A20 mAb produced by hybridoma cells. A20 mAb purified from the supernatant of a ncSFV cell line, or from the hybridoma, was conjugated to keyhole limpet hemocyanin and used to immunize Balb/c mice by administration of four weekly doses of 25 µg of mAb. Both idiotype mAbs were able to induce a similar antitumor protection and longer survival compared to non-immunized mice. These results indicate that the ncSFV RNA vector could represent a quick and efficient system to produce patient-specific idiotypes with potential application as lymphoma vaccines.
- Subjects :
- Idiotype
Protein vaccines
Lymphoma, B-Cell
medicine.drug_class
Expression systems
Science
Genetic Vectors
Mice, Nude
Cancer immunotherapy
Apoptosis
Alphavirus
Semliki Forest virus
Monoclonal antibody
Cancer Vaccines
Article
Mice
Immunoglobulin Idiotypes
hemic and lymphatic diseases
medicine
Tumor Cells, Cultured
Animals
Humans
B-cell lymphoma
Cell Proliferation
Mice, Inbred BALB C
Multidisciplinary
biology
Vaccination
Antibodies, Monoclonal
medicine.disease
biology.organism_classification
Virology
Xenograft Model Antitumor Assays
Cell culture
biology.protein
Medicine
Female
Antibody
Keyhole limpet hemocyanin
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....446ebaa346e32bdd089df17511c41aa8